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Immobilized Pepsin

Catalog Description Size Price(USD) Qty
786-791
Immobilized Pepsin 5ml Resin
$185.00

Immobilized Pepsin

Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)2 fragments from immunoglobulin G (IgG).  The pepsin has the ability to cleave the heavy chains near the hinge region. One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)2. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides.
The Immobilized Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)2 fragments.

 


The F(ab)2 fragments can be purified from undigested IgG with Immobilized Protein A and can be further purified from the small Fc fragments by dialyze with a 50kDa MWCO membrane (Tube-O-DIALYZER™) or by gel filtration.

 


Supplied as a 50% slurry in 50% glycerol, 0.1M sodium acetate, pH4.4 with sodium azide as a preservative.

 

Click here to request bulk or custom sizes.
 

 


Features

  • Generate F(ab)2 fragments
  • Eliminate contaminating pepsin enzyme
  • Can be used in virtually all scenarios using free pepsin

 

 

Protocol
786-791
Material Safety Data Sheet
786-791
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Technical Literature
Antibody Handbook
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
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