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Annexin V Apoptosis Assay (3 Citations)

Annexin-V-Apoptosis-Assay

Apoptosis is a form of programmed cell death which is essential as a normal part of development in multicellular organisms. Apoptosis go awry in some disease states such as Alzheimer’s and cancer.

 

Annexin V Apoptosis Assay contains fluorescent dye conjugated Annexin V  which detects apoptosis. It is based on a property of apoptotic cell where during onset of apoptosis, cells translocate the membrane phosphatidyl serine from inner face of plasma membrane to cell surface.  Annexin V protein has high binding affinity for phosphatidyl serine in presence of calcium ions. Thus Annexin V- fluorescent dye bound cell is apoptotic and can be quantified by FACS. Furthermore, propidium iodide is supplied in kit to differentiate apoptotic cells from necrotic cells.

*Alexa Fluor dyes is a registered trademark of Molecular Probes and now a part of Thermo Fisher Scientific

Features:

  • Designed to detect cells undergoing apoptosis
  • Available in 20 assay, 100 assays or 500 assays format with 100 μl assay volume.
  • Can differentiate between apoptotic and dead cells.
  • Suitable for flowcytometery and fluorescence microscope  based experiments.
  • Rapid:Staining can be done within 30 minutes.

 

Advantage(s): 

  • detect cells undergoing apoptosis
  • Can differentiate between apoptotic cells and necrotic cells as necrotic cells are stained with propidium iodide.
Protocol
786-1548
786-1548-350
786-1548-405
786-1548-488
786-1548-555
786-1548-594
786-1548-647
786-1543
786-1543-350
786-1543-405
786-1543-488
786-1543-555
786-1543-594
786-1543-647
786-1544
786-1544-350
786-1544-405
786-1544-488
786-1544-555
786-1544-594
786-1544-647
Material Safety Data Sheet
786-1548
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786-1543
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-1544
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish

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