Immobilized Biotin Resin (2 Citations)

Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.  The resin consists of biotin coupled to 6% cross-linked agarose. 

Biotin, a 244 Dalton vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵M^-1) and streptavidin.  Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH.  Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by boiling in SDS PAGE sample loading buffer.

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Features

• Strong affinity for avidin, streptavidin and neutravidin
• Reusable resin, at least 10 times
• Covalently coupled to limit leaching
• Spacer arm: 9Å

Applications

• Isolatin of avidin, streptavidin and Neutravidin coupled molecules
• Immunoprecipitations with avidin, streptavidin and Neutravidin coupled antibodies