Bacterial ProteaseArrest™ Protease Inhibitor Cocktail

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, Bestatin, E-64, EDTA, Pepstain A and PMSF that inhibits bacterial serine, cysteine and other bacterial specific proteases including aminopeptidases and aspartic proteases. The EDTA solution is provided in a spearate vial and is for enhanced metalloprotease inhibition. It is not present in the actual Bacterial ProteaseArrest^™ cocktail as it would inhibit the activity of proteins that require divalent cations (Ca²⁺, Mg²⁺ or Mn²⁺) for their biological activity. In addition, EDTA will inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC).

Features

• Broad spectrum bacterial specific protease inhibitor cocktail
• 100X concentrated, ready-to-use solution
• Versatile as a separate vial of EDTA, a metalloprotease, is provided to protect proteins that require metal ions or if IMAC is a downstream application
• No proprietary ingredients, fully disclosed formulation of six broad range protease inhibitors, including AEBSF, Bestatin, E-64, EDTA, Pepstain A and PMSF

Applications

• Inhibition of protease activity in protein preparations of bacterial lysates.
• Protection of proteins from proteolysis in such applications as electrophoresis, purification, storage, assays, and other applications.

ProteaseArrest Family