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CasPASE Apoptosis Colorimetric Assay™ (1 Citations)












The CasPASE™ Apoptosis Colorimetric Assay provides a simple and easy to follow method for assaying caspases, a key early indicator of apoptosis in mammalian cells.

The assay is based on the detection of cleavage of a synthetic substrate, which is labeled with the chromophore ρ-nitroaniline (ρNA) at the C-terminal. When liberated from the peptide, ρNA produces an optical change that can be detected by reading the absorbance at 405nm.

Comparison of the absorbance of an induced / apoptotic sample with an uninduced control allows one to determine the fold-increase in protease activity. 

Each CasPASE™ assay kit is supplied with necessary assay buffers and enzyme specific ρNA-substrate.

Fluorescent Caspase Assays are also available.

Features

  • Assays for caspase enzymes 2, 3,7,10, 6 and 9 are available
  • Colorimetric detection (405nm)
  • Conveniently adaptable for high-throughput 96-well format
  • All kits supplied with enzyme specific ρNA-substrate

Applications

  • Monitor apoptosis by measuring various caspase activities in cells and tissues
  • Use with purified enzyme preparations, cell extracts, and tissue lysates
  • Characterization of caspase enzymes
Protocol
BAQ001
BAQ002
BAQ003
BAQ007
BAQ008
BAQ009
BAQ013
BAQ014
BAQ015
BAQ019
BAQ020
BAQ021
Material Safety Data Sheet
BAQ001
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BAQ002
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BAQ003
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BAQ007
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BAQ008
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BAQ009
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BAQ013
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BAQ014
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BAQ015
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BAQ019
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BAQ020
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BAQ021
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