SOD Activity Assay
Superoxide dismutases (SODs) are metallo enzymes that catalyse the dismutation of the superoxide anion to molecular oxygen and hydrogen peroxide and thus form a crucial part of the cellular antioxidant defense mechanism.
Excessive reactive oxygen species, especially superoxide anion (O2•−), play important roles in the pathogenesis of many cardiovascular diseases, including hypertension and atherosclerosis. Superoxide dismutases (SODs) are the major antioxidant defense systems against O2•−, which consist of three isoforms of SOD in mammals: the cytoplasmic Cu/ ZnSOD (SOD1), the mitochondrial MnSOD (SOD2), and the extracellular Cu/ZnSOD (SOD3), all of which require catalytic metal (Cu or Mn) for their activation.
Superoxide Dismutase Activity Assay Kit (Colorimetric) is a sensitive kit using WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.
G-Biosciences Superoxide Dismutase Activity Assay kit utilizes a tetrazolium salt for detection of superoxide radicals generated by xanthine oxidase and hypoxanthine. One unit of SOD is defined as the amount of enzyme needed to exhibit 50% dismutation of the superoxide radical.
XOD and SOD Antagonism in the Generation of Formazan Dye. The conversion of xanthine and O2 to uric acid and H2O2 by XOD generates superoxide radicals. The superoxide anions reduce a tetrazolium salt (WST-1) to a colored formazan product (WST-1 formazan) that absorbs light. SOD scavenges superoxide anions, thereby reducing the rate of formazan dye formation.
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