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Oxidative Stress

Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect.

G-Biosciences Antioxidant Capacity Assays are useful for the determination of this parameter in different sample types such as food and beverages or plasma.

There are two types of mechanisms to determine the antioxidant capacity on different matrices: electron-transfer (E-T) methods, which measure the ability of an antioxidant to reduce an oxidant, with a change of color (ABTS, FRAP, DMPD) and hydrogen atom mediated (HAT) methods, which measure the ability of an antioxidant to eliminate free radicals through the donation of a hydrogen atom (they normally quantify peroxyl radicals; ORAC).

Biologic systems contain redox elements, which function in cell signaling, macromolecular trafficking and physiologic regulation. Oxidative stress includes disruption of this redox circuitry through altered functions of enzymes, receptors, transporters, transcription factors, and structural elements..
G-Biosciences’ ORAC assay depends on the free radical damage to a fluorescent probe, such as fluorescein, to result in a change of fluorescent intensity and the degree of change is indicative of the amount of radical damage. The presence of antioxidants results in an inhibition in the free rad..
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