DHE REDOX Probe

Cell permeant reagent Dihydroethidium (DHE) is a fluorogenic dye that is useful for the detection of reactive oxygen species (ROS). DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH-E+) (ex 500-530 nm/em 590-620 nm) or by non-specific oxidation by other sources of reactive oxygen species (ROS) to form ethidium (E+) (ex 480 nm/em 576 nm).

Reactive Oxygen Species (ROS) can be induced by stress conditions, such as exposure to oxidants or drugs. These then lead to oxidative stress. ROS induced damage in DNA, protein and lipids can lead to important consequences in cells.