3,3',5,5'-Tetramethylbenzidine, free base
3,3’,5,5’-Tetramethylbenzidine, free base, TMB,
MOLECULAR BIOLOGY GRADE
TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase and peroxidase coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.
Store at 2-8°C. Protect from light. Sensitive to moisture.
- CAS#: 54827-17-7
- Molecular Formula: C16H20ON2
- Molecular Weight: 240.35 g/mol