Proteinase K Assay [Colorimetric] is designed for rapid and reproducible quantification of Proteinase K activity. Proteinase K Assay [Colorimetric] is based on hydrolysis of denatured hemoglobin by proteinase K which releases Folin positive amino acids and peptides. The amino acids and peptide react with Folin’s reagent to give colored product which is measured at absorbance of 578nm.
Proteinase K is widely used in molecular biology for isolation and purification of nucleic acids from various tissue and cell samples. The proteolytic activity of proteinase K helps in removing nucleases from the sample, there by protecting DNA and RNA from degradation.
Recently, Proteinase K is widely used in COVID-19 virus diagnostic assays for processing samples such as saliva, nasopharyngeal and oropharyngeal swabs. After proteinase K treatment the samples are heat treated to inactivate proteinase K as well as inactivate any residual pathogens or viruses left in sample. Proteinase K assay can help in optimizing sample processing step for the COVID-19 diagnostic assay.
Features:
- Rapid and reproducible
- Linear detection range: 5µg/ml- 100µg/ml
- Available in 100 assay tube format, adaptable to microwell assay format.
Applications:
- Estimates proteinase K activity in various samples.
- Can be used in optimizing sample processing step for the COVID-19 diagnostic assay.
