LongLife™ Nuclease (13 Citations)

Catalog
Description
Size
Price(USD)
Qty
Catalog
786-039
786-039
Description
LongLife™ Nuclease [10U/μl]
LongLife™ Nuclease [10U/μl]
Size
0.5ml
0.5ml
$142.00
$142.00
Enzymes regularly used in laboratory applications often require preparation of fresh solution before each use. Making fresh enzyme solution for each application is time consuming and wasteful. A wide variety of enzyme preparations in a ready-to-use format are offered.
LongLife™ enzyme preparations have a long shelf life and no weighing or buffer preparation is needed; simply take an aliquot and add in your sample. LongLife™ enzyme preparations contain cofactors necessary for optimal enzymatic activity. Supplied in suspension form and when stored properly have a one year shelf life.
LongLife™ Nuclease is a cocktail preparation containing DNase (deoxyribonuclease) and RNase (Ribonucleases) as well as co-factors necessary for optimal activity. The LongLife™ Nuclease is ready-to-use solution that has a long shelf life (> 1 year). Simply add directly to your sample for high efficiency removal of RNA and DNA. LongLife™ Nuclease can be used in conjunction with protease inhibitor cocktails that do not contain EDTA, a nuclease inhibitor, e.g. FOCUS ProteaseArrest™.
A 1X LongLife™ Nuclease solution contains 80 units DNase and 1.2 Units RNase from bovine pancreas and 0.5mM cofactors.
All LongLife™ Enzymes Available:
LongLife™ Zymolyase® for digestion of yeast & fungal cell walls
LongLife™ Lysozyme for digestion of bacterial cell walls
LongLife™ Proteinase K for digestion of proteins in nucleic acid preparations
LongLife™ Nuclease for removal of nucleic acids
LongLife™ RNase for digestion of RNA
LongLife™ DNase for digestion of DNA
LongLife™ PE LB Lysozyme for digestion of bacterial cell walls & reduction of viscosity build-up due to
presence of nucleases (fully compatible with our PE LB™Systems)
Features
- Ready-to-use enzyme prepartion
- Contains DNase and RNase
- Compatible with protease inhibitor cocktails that do not contain EDTA
Applications
- High efficiency removal of DNA and RNA
- Lysis and extraction of nucleic acids
Protocol | |
786-039 | ![]() |
Material Safety Data Sheet | |
786-039 |
Technical Literature | |
Molecular Biology Handbook | A guide to our products for DNA and RNA. |
Protease & Phosphatase Inhibitors & Proteases Handbook | A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays |
Sample Preparation Handbook | For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment |
- Kamel, MM. et al (2023) LET-7B MICRORNA AND SIRTUIN-1 MRNA GENE EXPRESSION AS POTENTIAL BIOMARKERS FOR BREA. AIN SHAMS MEDICAL JOURNAL. 74:813.
- Evsyutina, Daria V. et al (2022) Gene Silencing throgh CRISPR Interference in Mycoplasmas. MICROORGANISMS. https://doi.org/10.3390/microorganisms10061159
- Lau, Su-Ee & Tan, Boon Chin (2021) Exogenous Nitric Oxide Promotes Growth and Enhances Tolerance against Drought Stress in Banana. BIO LIFE SCI FORUM.
- Silantyev, Artemiy S. et al (2021) Proteomic dataset: Profiling of glioma C6 and astrocytes rat cell lines before and after co-cultivation. DATA IN BRIEF. https://doi.org/10.1016/j.dib.2021.107658
- Zubov, A.I. et al (2021) Data on nucleoid-associated proteins isolated from Mycoplasma gallisepticum after intracellular infection. DATA IN BRIEF. https://doi.org/10.1016/j.dib.2021.107289
- Mouzykantov, A. et al (2020) Elsevier. doi.org/10.1016/j.dib.2020.106412
- O'Doherty, C. et al (2020) Characterisation and proteomic profiling of continuously exposed Cu-resistant variants of the Caco-2 cell line. Toxicology in Vitro. 65:104773
- O'Doherty, C. et al (2020) LC-MS proteomic profiling of Caco-2 human intestinal cells exposed to the copper-chelating agent, triethylenetetramine: A preliminary study. Biochemical & Biophysical Research Comm. 524(4):847-852
- Caruso, SM. et al (2019) A Novel Genus of Actinobacterial Tectiviridae. 11:1134
- Kamashev, D.E. et al (2019) Proteome of HU-Lacking E. coli Studied by Means of 2D Gel Electrophoresis. RUSS J BIOORG CHEM+. 45:366–373
- Kinoshita, S. et al (2019) Development of a Novel Platform of Proteome Profiling Based on an Easy-to-Handle and Informative 2D-DIGE System. BIOL PHARM BULL. 42:12
- Butcher, B.G. et al (2011) J Bacteriol 193:4598
- Markel, E. et al (2011) J Bacteriol 193:5775