Bioassays
Our Bioassays cover a wide range of proteomic and cell biology fields. Our CytoScan™ products offer a range of
non-radioactive bioassays for the study of chemical and cell-mediated cytotoxicity, viability, cell death or cell proliferation. Included are cell proliferation and cell toxicity assays which feature lactate dehydrogenase (LDH) assays, WST-1 assays and sulforhodamine B (SRB) assays. Our apoptotic line features caspase assays and a wide selection of inhibitors.
We offer continuous enzyme coupled assays for methylation that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
We have a phosphatase assay for the quantification of phosphatases and three phosphatase inhibitor cocktails to inhibit phosphatases and for phosphatase studies.
Gene/Protein:
A reliable colorimetric assay that measures the stable, cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells and is a biomarker for cellular cytotoxicity and cytolysis. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a t..
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Gene/Protein:
(9-fluoro-11β,17,21-trihydroxy-16a-methylpregna-1,4-diene-3,20-dione)
A potent and stable glucocorticoid steroid hormone that is often used as an anti-inflammatory and anti-rheumatic drug.
The mechanism of action is not fully understood, however it is thought that it may i..
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Gene/Protein:
Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect. Antioxidative effect includes protection of cells and cellular structures against the harmful effect of free radicals, especially oxygen and nitrogen. Substances with antioxidativ..
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Gene/Protein:
Malachite Green Phosphate Assay is a simple, rapid, colorimetric kit for estimation of free phosphate in the solution. The assay is based on the principle of quantifying the green color complex formed between malachite green, molybdate and free orthophosphate. The complex formation is rapid and can ..
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Gene/Protein:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
A highly sensitive, colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal pro..
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Gene/Protein:
Proteins may interfere with some assays, affecting accuracy and sensitivity. Chemical alternatives for removal of proteins are better option when ultrafiltration is not possible.
G-Biosciences PCA (Perchloric acid) Deproteinization Kit ensures a protein removal efficiency over 99% with very low s..
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Gene/Protein:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.
Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosy..
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Gene/Protein:
The SAM510™: SAM Methyltransferase Assay is a continuous kinetic enzyme coupled assay that can continuously monitor purified SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements. This is a sensitive enzyme-coupled assay for protein SAM memthyltransfer..
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Gene/Protein:
Proteins may interfere with some assays, affecting accuracy and sensitivity. Chemical alternatives for removal of proteins are better option when ultrafiltration is not possible.
G-Biosciences Sulfosalicylic Acid Deproteinization Kit ensures a protein removal efficiency over 98.3 % with low sampl..
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Gene/Protein:
Proteins may interfere with some assays, affecting accuracy and sensitivity. Chemical alternatives for removal of proteins are better option when ultrafiltration is not possible.
G-Biosciences TCA (trichloro acetic acid) Deproteinization Kit ensures a protein removal efficiency over 99 % with ver..
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Gene/Protein:
Proteins may interfere with some assays, affecting accuracy and sensitivity. Chemical alternatives for removal of proteins are better option when ultrafiltration is not possible.
G-Biosciences Zinc Sulfate Deproteinization Kit ensures a protein removal efficiency over 99 % with no sample dilution..
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Gene/Protein:
Contains 12 ready-to-use individual protease inhibitors for characterization of protease activity.
Each set contains the following protease inhibitors. See individual inhibitors for their specificities and other information.:
AEBSF
ALLN
Antipain, dihydrochloride..
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