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Biotechnology, Science for the New Millennium

G-Biosciences is the preferred vendor for Ellyn Daugherty's Biotechnology, Science for the New Millennium Program.

Biotechnology: Science for the New Millennium is a comprehensive program that prepares adults and teenagers for a variety of post-secondary options including community or career college biotechnology certificate programs, four-year biotechnology degree programs, and industry workplaces. The vast text and laboratory curriculum was developed and improved with input from an industry advisory committee composed of laboratory internship mentors. Biotechnology: Science for the New Millennium allows students to increase conceptual background as they build lab proficiencies. Science-bound and business-bound students benefit from the extensive skill development, science methods, and career exploration that permeate the curriculum.

Click here for Ellyn's recommended reagents list

Biotechnology Basics™ by Ellyn Daugherty

  1. Cheese Biotechnology: Cheese Production: The Evolution of Cheese-Making Technology (Lab 1C)
  2. DNA Spooling with a Twist: Genomic DNA Spooling (Lab 4B)
  3. Micropipetting Mastery: Micropipetting: Measuring Small Volumes in Biotechnology (Lab 3B)
  4. Gel Box Science (Lab 4J)
  5. Crime Scene DNA Analysis using RFLP Technology (Lab4M)
  6. GFP Genetic Engineering of Bacteria (Lab 4N)

Biotechnology: Science for the New Millennium (BS4NM): The rAmylase Project Kits

  1. Characterizing Proteins by PAGE (Lab 5F)
  2. Assaying for Amylase Activity (Lab 6D)
  3. Direct ELISA of Bacterial α-Amylase (Lab 6E)
  4. Western Blot to Identify α-Amylase (Lab 6G)
  5. Determining the Concentration of Amylase in a Solution (Lab 7D)
  6. Restriction Digestion to Verify the pAmylase Plasmid (Lab 8B)
  7. Transformation of E. coli with pAmylase (Lab 8C)
  8. Using A Miniprep Kit for Plasmid Isolation (Lab 8G)
  9. Using Ion Exchange Chromatography to Separate Proteins (Lab 9C)
  10. Confirming the Amylase Gene Using PCR (Lab 13H)

Other Biotechnology: Science for the New Millennium (BS4NM) Kits

  1. Bacterial Gram Staining (Lab 4L)
  2. Genomic DNA Isolation (Lab 4H)

  Download the Handbook for All Our Science Education Products

Gene/Protein:
This Biotechnology Basics™ by Ellyn Daugherty is designed to teach students the basic principles of measuring small volumes (<500µl) using micropipettes. The kit is supplied with all the reagents to allow 8 groups of 4 students to learn about P-1000, P-200 or P-100 and P-20 or P-10 mi..
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Amylase producing plasmid for Ellyn Daugherty Biotechnology:Science for the New Millennium course. See the figure for key restriction and structural elelments of the plasmid...
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This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to: Set up restriction digests of the p-Amylase plasmid with two enzymes Set up positive and negative controls for the rest..
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This kit is designed to teach 8 groups of four students the principles of bacterial transformation.   The kit contains the reagents to allow students to: Generate E.coli comptent cells Transform competent cells with the p-Amylase plasmid Grow transformed colonies on selection plates of L..
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This kit is designed to teach 8 groups of four students the principles of plasmid DNA purification by minipreps.   The kit contains the reagents to allow students to: Collect p-Amylase-transformed bacteria from an overnight culture Resuspend bacteria, lyse and isolate the p-Amylase plasmi..
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This kit is designed to teach 8 groups of four students the principles of Western blotting.   The kit contains the reagents to allow students to: Conduct a serial dilution of a stock amylase solution Prepare samples for PAGE electrophoresis Separate proteins by electrophoresis Transfer ..
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This kit is designed to teach 8 groups of four students the principles of Ion Exchange Chromatography and protein purification.  The kit contains the reagents to allow students to: Equilibrate a DEAE agarose column Load a protein sample on the column (amylase, lysozyme or a mixture of both)..
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Gene/Protein:
This kit is designed to teach 8 groups of four students the principles of enzyme assays.   The kit contains the reagents to allow students to test for the presence and activity of 2 different types of amylase by: Visualizing the breakdown of the starch substrate by the amylase enzym..
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