Mouse Brain Tissue Region Blot (1 Citations)
The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse brains.
The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer.
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.
Blot lane order:
- Protein marker
- Mouse Frontal Cortex Lysate
- Mouse Posterior Cortex Lysate
- Mouse Cerebellum Lysate
- Mouse Hippocampus Lysate
- Mouse Olfactory Bulb Lysate
- Mouse Striatum Lysate
- Mouse Thalamus Lysate
- Mouse Midbrain Lysate
- Mouse Entorhinal Cortex Lysate
- Mouse Pons Lysate
- Mouse Medulla Lysate
- Mouse Spinal Cord
- Mouse Whole Brain Lysate