RNase Inhibitor

RNase Inhibitor is a protein-based ribonuclease inhibitor which noncovalently binds and inactivates a wide variety of RNases in a range of temperature (37–65°C) and pH (5.5–8.5) conditions. This product is distinct from placental RNase Inhibitor protein in that it inactivates RNases I and T1 in addition to RNases A, B and C. EZ2023 is distinct from RNase Inhibitor protein in that it is less expensive, have more robust interactions with RNAses, and do not release active RNases in the absence of DTT or other reducing agents. EZ2023 does not interfere with the activities of SP6, T7, and T3 RNA Polymerases, MMLV Reverse Transcriptase, or Taq DNA Polymerase.

Applications: RNase Inhibitor is used at a final concentration of 1-10 U/μL to prevent RNA degradation in applications including cDNA synthesis, RT-PCR, in vitro transcription, in vitro translation, preparation of cell lysates, RNA isolation and storage, and in any application where ribonuclease inhibitor protein is used. In experiments requiring intact RNA, (e.g., transcription), avoid denaturation of the Atto RNase Inhibitor protein. The addition of DTT is not recommended for storage.