CB™ Protein Assay

CB™ Protein Assay

An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from brown to blue. The change in color density is proportional to protein concentration. Protein estimation can be performed using as little as 0.5µg protein

CB™ Protein Assay is supplied with a simple to follow protocol. Ready to use reagent that does not require prefiltering or dilution. Simply mix the protein solution with CB™ Protein Dye and read optical density.

The protein-dye complexes reach a stable end point in 5 minutes. The CB™ Protein Assay is compatible with reducing agents and a wide variety of common laboratory agents listed below.
 
The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.

Note: The Coomassie dye based assay is not suitable if the protein solution contains higher than recommended concentration of detergents or other agents (see Table 1).
 
Tolerance to Common laboratory Agents
The following table kists the agents compatible with the CB™ Protein Assay. Table 1 also shows the acceptable concentration of reagents for standard protocols.  In most cases, using a correct blank will eliminate or minimize the error caused by interference.
 
Table 1: A selection of compounds and the maximum concentrations that are compatible with the CB™ Assay.
Compound Concentration Compound Concentration Compound Concentration
Amino acids 1mM DTT 1M Phenol 5%
Ammonium sulfate 1M EDTA 100mM Sodium azide 0.5%
Ampholytes 0.5% EGTA 50mM Sodium chloride 6M
Ascorbic acid 50mM Ethanol 10% Sodium dodecyl sulfate (SDS) 0.015%
Boric acid 1mM Glucose 1M Sodium hydroxide 0.1M
Brij® 35 0.06% Glycerol 10% Sodium phosphate 0.1M
CHAPS 0.5% Glycine 0.1M Sucrose 25%
CHAPSO 0.5% Guanidine.HCl 6M Tris 2M
Citrate 0.05% HEPES 0.1M Triton® X-100 0.06%
Cysteine 10mM 2-mercaptoethanol 1M tRNA 0.35mg/ml
Deoxycholate 0.1% Methanol 10% Tween® 20 0.03%
DMSO 10% MES 0.7M Urea 3M
DNA 1mg/ml Nonidet® P-40 0.5%
 

 

 

 


Features

  • Sensitivity: Linear responses over the range of 0.5µg-50µg protein
  • Flexible Protocols: Suitable for tube or Titer plate assays
  • Ready to use assay reagents and no preparation required
  • Long shelf life, stable for 12 months

 


Applications

  • Suitable for non-detergent solubilized proteins.
  • Protein estimation in protein purification, electrophoresis, cell biology, molecular biology, and other research applications.
  • Suitable for protein samples containing common laboratory agents.

 

 

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Protocol
786-012
786-012T
786-893
Material Safety Data Sheet
786-012
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786-012T
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-893
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Technical Literature
A Non-Animal Alternative to BSA Protein Standards
Bioassays Handbook
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
Catalog Description Size Price(USD) Qty
786-012
CB™ Protein Assay with Albumin Standard 500 Assays
$78.00
786-012T
CB™ Protein Assay Trial 15 Assays
$20.00
786-893
CB™ Protein Assay with Non Animal Protein Standard 500 Assays
$78.00