Free radicals are highly reactive species that are naturally formed during cell metabolism in the mitochondria, secretion by immune cells during inflammatory processes or taken up as environmental pollutants.
CUPRAC (CUPric Reducing Antioxidant Capacity) Assay is a new method, which enables the Total Antioxidant Capacity (TAC) measurements of hydrophilic as well as hydrophobic samples. The main reagent, the copper (II)-neocuproine (2,9-dimethyl-1,10-phenanthroline), can oxidize antioxidants generating a colored product. The chelation with neocuproine enables a faster reaction by elevating the redox potential of the reagent.
G-Biosciences’ CUPRAC Antioxidant Capacity Assay Kit is a stable, selective, easy and quick assay that measures all of the significant antioxidants on biological samples at physiological pH.
The CUPRAC assay is a redox reduction between the CUPRAC reagent and the antioxidants with a leading thiol group (like for example glutathione) present in the sample. In this process, the reagent reduces itself forming a chelate complex of copper (I)-neocuproine, which provides a color measurable at 450 nm.
