CasPASE Apoptosis Colorimetric Assay™

CasPASE Apoptosis Colorimetric Assay™

The CasPASE™ Apoptosis Colorimetric Assay provides a simple and easy to follow method for assaying caspases 1-10, a key early indicator of apoptosis in mammalian cells.

 

The assay is based on the detection of cleavage of a synthetic substrate, which is labeled with the chromophore ρ-nitroaniline (ρNA) at the C-terminal. When liberated from the peptide, ρNA produces an optical change that can be detected by reading the absorbance at 405nm.

 

The reaction is selectively and irreversibly inhibited by the peptide Z-VAD-FMK. Comparison of the absorbance of an induced / apoptotic sample with an uninduced control allows one to determine the fold-increase in protease activity. The Caspase activity can also be quantitated by using a standard curve established with the free ρNA dye.

 

CasPASE™ assays are available for caspase enzymes 1 through 10. Each CasPASE™ assay kit is supplied with necessary assay buffers, enzyme specific ρNA-substrate, free dye (ρNA), and the potent caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.

 

Fluorescent Caspase Assays are also available.

 


Features

  • Assays for caspase enzymes 1 through 10 are available
  • Colorimetric detection (405nm)
  • Conveniently adaptable for high-throughput 96-well format
  • All kits supplied with enzyme specific ρNA-substrate, free dye (ρNA) & inhibitor Z-VAD-FMK

 


Applications

  • Monitor apoptosis by measuring various caspase activities in cells and tissues
  • Use with purified enzyme preparations, cell extracts, and tissue lysates
  • Characterization of caspase enzymes

 

 

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Protocol
786-858A
786-858B
786-859A
786-859B
786-860A
786-860B
786-861A
786-861B
786-862A
786-862B
786-863A
786-863B
Material Safety Data Sheet
786-858A
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English_UK
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786-858B
English_US
Dutch
English_UK
French
German
Portuguese
Swedish
786-859A
English_US
Dutch
English_UK
French
German
Portuguese
Swedish
786-859B
English_US
Dutch
English_UK
French
German
Portuguese
Swedish
786-860A
English_US
Dutch
English_UK
French
German
Portuguese
Swedish
786-860B
English_US
Dutch
English_UK
French
German
Portuguese
Swedish
786-861A
English_US
Dutch
English_UK
French
German
Portuguese
Swedish
786-861B
English_US
Dutch
English_UK
French
German
Portuguese
Swedish
786-862A
English_US
Dutch
English_UK
French
German
Portuguese
Swedish
786-862B
English_US
Dutch
English_UK
French
German
Portuguese
Swedish
786-863A
English_US
Dutch
English_UK
French
German
Portuguese
Swedish
786-863B
English_US
Dutch
English_UK
French
German
Portuguese
Swedish


Catalog Description Size Price(USD) Qty
786-858A
CasPASE™ 1, 4, 5 assay with Ac-WEHD-pNA substrate 50 Assays
$260.00
786-858B
CasPASE™ 1, 4, 5 assay with Ac-WEHD-pNA substrate 100 Assays
$401.00
786-859A
CasPASE™ 2 assay with Ac-VDVAD-AFC pNA substrate 50 Assays
$260.00
786-859B
CasPASE™ 2 assay with Ac-VDVAD-AFC pNA substrate 100 Assays
$401.00
786-860A
CasPASE™ 3, 7, 10 assay with Ac-DEVD-pNA substrate 50 Assays
$260.00
786-860B
CasPASE™ 3, 7, 10 assay with Ac-DEVD-pNA substrate 100 Assays
$401.00
786-861A
CasPASE™ 6 assay with Ac-VEID-pNA substrate 50 Assays
$260.00
786-861B
CasPASE™ 6 assay with Ac-VEID-pNA substrate 100 Assays
$401.00
786-862A
CasPASE™ 8 assay with Ac-LETD-pNA substrate 50 Assays
$260.00
786-862B
CasPASE™ 8 assay with Ac-LETD-pNA substrate 100 Assays
$401.00
786-863A
CasPASE™ 9 assay with Ac-LEHD-pNA substrate 50 Assays
$260.00
786-863B
CasPASE™ 9 assay with Ac-LEHD-pNA substrate 100 Assays
$401.00