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Alamar Blue Cell Viability Assay (7 Citations)



AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye in the Alamar blue assay incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes color in response to the chemical reduction of growth medium due to cell growth.
 
The alamarBlue (resazurin) dye in its oxidized form is blue in color and non-fluorescent. In alamarBlue assay the growing cells cause a chemical reduction of the alamarBlue dye from non-fluorescent blue to red fluorescent. The continued growth of viable cells maintain a reducing environment (fluorescent, red) and inhibition of growth maintains an oxidized environment (non-fluorescent, blue), which can be detected using a fluorescence or absorbance detector.

 


Features

  • High sensitivity and linerarity
  • Quantitative
  • Non-toxic to cells
  • Scalable
  • No cell lysis
  • Stable
  • Flexible and versatile

 


Applications

  • Monitor cell proliferation and cell viability for in vitro cytotoxicity studies (e.g., effects of environmental toxins, cytokines, drugs, siRNA/microRNA)
  • Monitor cell viability in real time for 3D tissue engineering applications

 

 

 
Protocol
786-921
786-922
786-923
Material Safety Data Sheet
786-921
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
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Polish
786-922
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-923
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Certificate Of Analysis
786-921
190212
190808
212010
202107
786-922
212010
786-923
162405
190212
190808

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