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Glutathione Assay (Colorimetric)

Catalog Description Size Price(USD) Qty
786-075
Glutathione Assay (Colorimetric) 225 assays
$313.00
786-076
Glutathione Assay (Colorimetric) 450 assays
$480.00

Glutathione Assay (Colorimetric)

Reduced glutathione (L-γ-glutamyl-L-cysteinylglycine), a tripeptide, serves as a key antioxidant in animal, plant, fungi and bacteria by providing free thiol. Glutathione exist in reduced (GSH) and oxidized (GSSG; gluthathione disulphide) forms in cells and tissues, and the concentration of glutathione range from 0.5 to 10mM in animal cells1. The majority (90-95 %) of glutathione exist in reduced form (GSH) in healthy cells. GSH provides reducing equivalents to antioxidant enzymes, hydroxyl radicals, ROS and is itself oxidized to GSSG. Therefore GSH/GSSG ratio is critical indicator of the health of cell. During oxidative stress there is decrease in levels of GSH and increase in levels of GSSG and thus GSH/GSSG ratio decreases.

 

Glutathione colorimetric assay kit is designed to measure reduced glutathione (GSH), oxidized glutathione (GSSG) and total glutathione (GSH+GSSG) concentrations in wide range of samples like, blood, plasma, serum, cultured cells and tissue. The assay involves carefully optimized enzymatic recycling method using glutathione reductase and Ellman’s reagent (DTNB) 2.

 

 


Features

  • Measure total, oxidized (GSSG) and reduced (GSH) glutathione colorimetrically 
  • Assay Range: 0.1-2.5 μM for GSSG and for GSH it would be 0.2-5 μM
  • Plated based, colorimetric measurement at 405-415nm
  • End-point and kinetic methods
  • 225 and 450 assay sizes available
  • The assay is designed to measure glutathione in wide range of samples such as plasma, blood, serum, urine, cultured cells, tissue etc. 

 


Applications

  • Total Glutathione Colorimetric assay kit measures total, oxidized and reduced glutathione in wide range of samples such as blood, plasma, serum, cultured cells and tissues
  • Estimating glutathione in cells is one of the ways to measure oxidative stress and this assay is also vital for mitochondrial toxicity screening
  • GSH/GSSG ratio decreases in many pathological diseases such as cancer, diabetes, cardiovascular disorders, artherosclerosis, Parkinson’s and Alzheimer’s3, 4 and thus estimating glutathione is vital in this area of research.  

 

 

Protocol
786-075
786-076
Material Safety Data Sheet
786-075
English_US
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English_UK
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786-076
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
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