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JC-10 Mitochondrial Membrane Potential Assay

Catalog Description Size Price(USD) Qty
786-1541
JC-10 Mitochondrial Membrane Potential Assay 100 assays
$108.00
786-1549
JC-10 Mitochondrial Membrane Potential Assay 500 assays
$438.00

JC-10 Mitochondrial Membrane Potential Assay

Mitochondrial membrane potential is one of the key parameters of mitochondrial function and serves as an indicator of cell health.

JC-10 Mitochondrial Membrane Potential Assay is based upon JC-10 dye, which is a superior alternative to JC-1 dye due to its increased solubility. JC-10 shows greater solubility even at higher concentrations. JC-10 dye, like JC-1, is a cationic, lipophilic dye that gets concentrated in healthy mitochondria to form reversible red-fluorescent JC-10 aggregates (Ex/Em=540/590nm). In apoptotic cells, the mitochondrial membrane potential collapses, which results in failure to retain JC-10 dye and the dye returns to its monomeric green form (EX/Em= 490/525 nm) in the cytosol.

Feature(s)

  • Measures the membrane potential of mitochondria
  • Contains JC-10 dye which is superior to JC-1 dye due to its increased solubility
  • available  100 or 500 microplate assay formats. 
  • Compatible with microscopy and flow cytometry assay.
  • Stains only live cells
  • Rapid staining : 15 min-1hr

Applications

  • Measures cell health by measuring the mitochondrial membrane potential
  • Can be used to monitor apoptosis or check apoptotic inducers and inhibitors.
Protocol
786-1541
786-1549
Material Safety Data Sheet
786-1541
English_US
786-1549
English_US
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