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TMRE Mitochondrial Membrane Potential Assay

Catalog Description Size Price(USD) Qty
786-1313
TMRE Mitochondrial Membrane Potential Assay 100 tests
$90.00
786-1314
TMRE Mitochondrial Membrane Potential Assay 500 tests
$267.00

TMRE Mitochondrial Membrane Potential Assay

TMRE Mitochondrial Membrane Potential Assay quantifies changes in mitochondrial membrane potential of live cells via microplate fluorometry, fluorescence microscopy and flow cytometry.

Tetramethylrhodamine, ethyl ester (TMRE) is a cell permeable, positively charged red-orange dye that accumulates in active mitochondria due to their relative negative charge. Inactive or depolarized mitochondria have decreased membrane potential and thus fail to retain the TMRE dye and as a result show low fluorescence signal.

TMRE is suitable for staining mitochondria in live cells only. It does not work with fixed cells.

Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) induced depolarization in mitochondria and eliminates mitochondrial membrane potential.
FCCP is a potent mitochondrial oxidative phosphorylation uncoupler and serves as positive control for depolarization of mitochondrial membrane or for apoptosis.

 

Feature(s):

  • TMRE Mitochondrial Membrane Potential Assay quantifies changes in mitochondrial membrane potential of live cells
  • Contains TMRE, FCCP control and the assay buffer.
  • 100 tests or 500 test format for microwells avaialble.
  • Stain only live cells and does not work on fixed cells.
  • Suitable for fluorescence microscopy, fluorometry or flow cytometry.
  •  Rapid staining: Cells with active mitochondria can be stained  with TMRE in ≤ 30 min.



Application(s)
  • TMRE Mitochondrial Membrane Potential Assay quantifies changes in mitochondrial membrane potential of live cells via microplate fluorometry, fluorescence microscopy and flow cytometry.
Protocol
786-1313
786-1314
Material Safety Data Sheet
786-1313
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786-1314
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
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