Malondialdehyde (MDA) TBARs Assay

Malondialdehyde (MDA), can be generated by oxidizing agents that alters lipid structure, creating lipid peroxides. MDA can be measured as Thiobarbituric Acid Reactive Substances (TBARS).

This method, is convenient to determine the relative lipid peroxide content of samples, including plasma, serum, cell culture supernatants and urine samples. Multi-unsaturated lipids are most likely to form peroxides, and so they are the most reactive lipids in the TBARs assay. TBARs assay is commonly used to compare one set of samples to another.

Lipid oxidation is reported to increase with age and correlates with some clinical features of cardiovascular disease, ischemia/reperfusion and cerebrovascular disorders among others.

The Thiobarbituric Acid Reactive Substances (TBARS) Assay Kit is a tool for the direct quantitative measurement of MDA in biological samples. The samples containing MDA or the MDA standards are reacted with TBA.

After the incubation, the samples and standards can be read spectrophotometrically or fluorometrically.

The MDA concentration in the samples is then calculated by comparison with the MDA standard curve.