Trypan Blue 0.4% Solution is commonly used as a cell stain to measure cell viability using dye exclusion method. The dye exclusion method is based on the principle that cell impermeable dyes like Trypan blue will stain only dead cells where as viable cells will not be stained.
Trypan Blue staining is routinely used when counting cells with hemocytometer during subculturing of cells. It can also be used anytime when cell viability need to be determined accurately and quickly.
- Solution is made in PBS, pH 7.4 ± 0.1
- Sterile filtered
- Store at ambient temperature
- Trypan Blue 0.4% Solution is routinely used for counting cells with hemocytometer during subculturing of cells.
- It can be used to check cell viability accurately and quickly anytime