menu
Your Cart

4-Vinylpyridine

Catalog Description Size Price(USD) Qty
786-031
4-Vinylpyridine 1 ml
$31.00

4-Vinylpyridine

4-Vinylpyridine is used as a derivatizing reagent for free thiols such as GSH. It is used in the Glutathione colorimetric assay to remove reduced GSH, so that the oxidized Glutathione (GSSG) concentration can be measured. 4-Vinylpyridine is a better derivatizing reagent for GSH when compared to N-ethyl-maleimide (NEM) as N-ethyl-maleimide is a potent inhibitor of glutathione reductase. The treatment of samples with 4-vinylpyridine removes all the free thiols present in the sample leaving only GSSG which can be quantified in the same way as total glutathione using Ellman’s Reagent.  

 

4-Vinylpyridine alkylates cysteine and cystine residues (after reduction) in proteins to give derivatives that are stable to acid hydrolysis and so it is used in analysis of proteins2. Its alkylating property also enables it to be used for preparation of proteins from PAGE for peptide mapping by MALDI-MS and MALDI-TOF.

*  This product is shipped only within US   
 

Features

  • 4-Vinylpyridine supplied in low volumes. This feature enables better handling of this flammable product. In addition for derivatization of GSH for oxidized glutathione assay, low concentrations of 4-Vinylpyridine are needed.
  • It can be used as derivatizing agent for free thiols or alkylating agent depending upon requirements.
  • Molecular formula: C7H7N
  • Molecular weight: 105.1
  • CAS #: 100-43-6
  • Density: 0.975 g/ml

 


Applications

  • Removes GSH in samples so that oxidized glutathione concentration can be measured.
  • It can be used in protein structure analysis as it alkylates cysteine and cystine residues (after reduction) in proteins to give derivatives that are stable to acid hydrolysis.
  • It can be used for preparation of proteins from PAGE for maximal recovery for peptide mapping by MALDI-MS and MALDI-TOF.

 

 

Protocol
786-031
Material Safety Data Sheet
786-031
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Write a review
Note: HTML is not translated!
Bad Good
Captcha

Download our handbooks

Custom Services

Connect With Us

Stay up to date with G-Biosciences by signing up for our newsletter.