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Cross-Linking & Modification

Download the Crosslinkers Handbook & Selection Guide            Download the Protein Labeling & Conjugation Handbook

 

 Cross-linking agents contain at least two reactive groups that are reactive towards numerous groups, including sulfhydryls, amines and carbohydrates, and create chemical covalent bonds between two or more molecules.  functional groups that can be targeted with cross-linking agents are primary amines, carboxyls, sulfhydryls,  carbohydrates and carboxylic acids. Protein molecules have many of these functional groups and therefore proteins and peptides can be readily conjugated using cross-linking agents. Cross-linking agents are used to study protein structure and function, to anchor proteins to solid  supports, preparation of immunogens, immunotoxins, and other conjugated protein reagents. G-Biosciences offers a variety of Double Do™ cross-linking agents, reaction specific buffers, and accessories for performing and facilitating crosslinking applications. These accessory tools simplify cross-linking reactions and will enable researchers to achieve highly efficient cross-linking reactions with minimal effort in reaction optimization, set-up, and procedure development

 
We offer a variety of amino acid side chain modifiers to block side chain interactions, change the charge or modify them to be more favorable for other reactions. Many denaturants, or chaotropes, are available and can disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. Chaotropes act on proteins to unfold them and alter their three-dimensional structure. A wide selection of reagents and accessories are also available.
1,4 Dithiothreitol (DTT) is a small molecule reducing agent, also known as Cleland’s reagent. G-Biosciences DTT is a high quality proteomic grade DTT and is supplied in various sizes, including a OneQuant™ version. The OneQuant™ format features 40 vials of 7.7mg DTT for conven..
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EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and i..
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Ethylene glycol bis(succinimidyl succinate)   Ideal for receptor ligand crosslinking   Features Molecular Weight: 456.36 Spacer Arm (Å): 16.1 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: Hydroxylamine   ..
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5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)   Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colore..
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N-(ε-Maleimidocaproic acid) hydrazide   Features Molecular Weight: 225.24 Spacer Arm (Å): 11.8 Reactive Toward: Sulfhydryl + Carbohydrate Membrane Permeable: Not tested  Water Soluble: NO Cleavable/ Reversible: NO    ..
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N-(ε-Maleimidocaproyloxy) succinimide ester   Features Molecular Weight: 308.29 Spacer Arm (Å): 9.4 Reactive Toward: Primary Amine + Sulfhydryl Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: NO    ..
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N-γ-Maleimidobutyryloxysuccinimide ester     Features Molecular Weight: 280.23 Spacer Arm (Å): 6.8 Reactive Toward: Primary Amine + Sulfhydryl Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: NO    ..
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A Solid Phase Iodination Reagent Radioactive iodine (125I) is routinely used by researchers to label proteins. The iodination of proteins can be performed enzymatically or chemically. The Iodination reagent isdesigned to aid in the labeling of proteins with radioactive iodine.   The iodinati..
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A proteomic grade iodoacetamide in two different quantities. Iodoacetamide is supplied in 5gm bulk quantities or in the OneQuant™ format.   OneQuant™ Iodoacetamide OneQuant™ iodoacetamide are single aliquots of iodoacetamide that eliminate the need for weighing, preventing ..
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Maleimide PEG succinimidyl carboxymethyl   Features Molecular Weight: ~3400 Reactive Toward: Primary Amine + Sulfhydryl  Membrane Permeable: Not tested  Water Soluble: NO Cleavable/ Reversible: NO    ..
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m-Maleimidobenzoyl-N-hydroxysuccinimide ester   Ideal for hapten-carrier protein, toxin-antibody, enzyme-antibody crosslinking   Features Molecular Weight: 314.25 Spacer Arm (Å): 9.9 Reactive Toward: Primary Amine + Sulfhydryl  Membrane Permeable: YES ..
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N-Hydroxysuccinimidyl-4-azidosalicyclic acid   Features Molecular Weight: 276.21 Spacer Arm (Å): 8.0 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: NO    ..
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