CytoScan™ LDH Cytotoxicity Assay

CytoScan™ LDH Cytotoxicity Assay

The CytoScan™ LDH Cytotoxicity Assay is a colorimetric method of assaying cellular cytotoxicity and is an alternative to 51Cr release cytotoxicity assays. The kit combines the advantages of reliability and simple evaluation, characteristic of radioisotope release assays, with the convenience of accuracy and avoidance of radioactivity. The CytoScan™ LDH Cytotoxicity Assay Kit can be used with different cell types for assaying cell mediated cytotoxicity as well as cytotoxicity mediated by chemicals and other test compounds.

 
The assay quantitatively measures the stable, cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a tetrazolium salt (iodonitrotetrazolium (INT)) into a red color formazan by diaphorase. The LDH activity is determined as NADH oxidation or INT reduction over a defined time period.
 
The resulting formazan absorbs maximally at 492nm and can be measured quantitatively at 490nm.

 

Features

  • Quantitatively measures LDH release
  • Colorimetric assay
  • Ideal for high throughput screening

 


Applications

  • For the detection of cell toxicity, death, viability or proliferation
  • For cell free supernatants from cells in culture (adherent or suspension) 

 

 

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Protocol
786-210
786-324
Material Safety Data Sheet
786-210
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786-324
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Technical Literature
Bioassays Handbook
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
Catalog Description Size Price(USD) Qty
786-210
CytoScan™ LDH Cytotoxicity Assay 1000 Assays
$300.00
786-324
CytoScan™ LDH Cytotoxicity Assay 200 Assays
$65.00