CytoScan™ WST-1 Cell Proliferation Assay

CytoScan™ WST-1 Cell Proliferation Assay

CytoScan™ WST-1 Cell Proliferation Assay is a sensitive and accurate assay for cell proliferation and cytotoxicity. The assay is highly convenient as it is performed in a single cell tissue culture well and requires no washing, harvesting or solubilization of cells.  

 

Adherent or suspension cells are cultured in a microplate and then incubated with WST-1 and the assay is monitored with a spectrophotometer. The assay principle is based upon the reduction of the tetrazolium salt WST-1 to formazan by cellular dehydrogenases. The generation of the dark yellow colored formazan is measured at 420-480nm (optimal at 440nm) and is directly correlated to cell number.

 

The kit components are sufficient for performing up to 500 or 2,000 assays.

 


Features

  • Uses WST-1, a high sensitivity tetrazolium salt.
  • Colorimetric assay.
  • Adherent or suspension cells.
  • No washing, harvesting or solubilization required.

 


Applications

  • For the detection of cell toxicity, death, viability or proliferation.
  • Ideal for high throughput screening.

 

 

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Protocol
786-212
786-857
Material Safety Data Sheet
786-212
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786-857
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Technical Literature
Bioassays Handbook
Comparison of Two Colorimetric Assays in the Detection of Cytotoxicity in Fibroblasts Initiated by Apoptotic Inducers Comparison of Two Colorimetric Assays in the Detection of Cytotoxicity in Fibroblasts Initiated by Apoptotic Inducers
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
Catalog Description Size Price(USD) Qty
786-212
CytoScan™ WST-1 Cell Cytotoxicity Assay 500 Assays
$146.00
786-857
CytoScan™ WST-1 Cell Cytotoxicity Assay 2000 Assays
$553.00