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DetergentOUT™ GBS10 (9 Citations)






Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins, and suppress peptide ionization when analyzed by mass spectrometry.

 

DetergentOUT™ GBS10 features a research grade detergent binding resin that has a high affinity for most detergents and a low affinity for proteins and peptides. DetergentOUT™ GBS10 is ideal for detergent removal prior to ELISA, IEF, protease digestion of proteins, and peptide ionization when analyzed by mass spectrometry.

 

DetergentOUT™ GBS10 is available in four spin format column sizes or as resin alone.  The column specifications are:

Cat. # Description Sample Size (µl) Resin Volume (µl)
786-154 DetergentOUT™ GBS-10-125 10-30 125
786-155 DetergentOUT™ GBS-10-800 30-200 800
786-156 DetergentOUT™ GBS-10-3000 200-750 3,000
786-157 DetergentOUT™ GBS-10-5000 500-1,250 5,000

 

Our DetergentOUT™ GBS10 resin removes free, unbound anionic, nonionic or zwitterionic detergents (e.g. SDS, Triton® X-100 or CHAPS) from aqueous protein and peptide samples with minimal sample loss (Table below) for downstream analysis by mass spectrometry (Figure 1 & 2) and other techniques.

 

Also available in 96-well spin plate formats for processing up to 96 samples.

 

The DetergentOUT™ GBS10 columns were shown in independent studies to be fully compatible with DI-QTOF and LC-MS/MS (see references). The use of the DetergentOUT™ GBS10 columns significantly increased the number of peptide spectra detected (Figure 1 & 2).

 

In addition, the DetergentOUT™ GBS10 columns have a high binding capacity for detergents, with 6 milligrams SDS (Figure 3) and 14 milligrams Triton® X-100 for every milliliter of settled resin.

 

 Detergent % Removed BSA Phosphorylase B  Cytochrome C  E.coli Lysate
 Triton X-100, 2%  >99  >90  >91  >92  >93
 Triton X-114, 2%  >96  >99  >98 >97  >91
 Nonidet P-40, 1%  >96  >93  >95  >91  >91
 Brij 35, 1%  >99  >98  >99  >97  >91
 SDS, 2.5%  >99  >96  >97  >92  >90
 Sodium deoxycholare, 5%  >99  >99  >99  >98  >95
 CHAPS, 3%  >99  >92  >95  >92  >91
 Octyl glucoside, 5%  >99  >93  >95  >96  >91
 Lauryl maltoside, 1%  >97  >99  >99  >99  >91
 Tween 20*, 0.25%  >98  >86  >85  >89  >85
 Tween 80*, 0.13%  >85  >83  >81  >80  >81

Detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10

 

 


Features

  • Easy-to-use, spin-format columns
  • Rapid removal of free detergents
  • Suitable for anionic, non-ionic and zwitterionic detergents
  • Minimal sample loss
  • Available for sample volumes ranging from 10µl to 1,250µl

 


Applications

  • Detergent removal from protein and peptide solutions
  • Detection of peptide fragments by Mass spectrometry
  • Enhances Mass spectrometry Spectra
  • Ideal for Mass spectrometry sample preparation and prior to downstream analysis and applications

 

 

Protocol
786-154
786-155
786-156
786-157
786-159
786-998
Material Safety Data Sheet
786-154
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-155
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-156
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-157
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-159
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-998
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
ASMS Poster, May 2010 Alvarez, S. et al. Efficiency assay of detergent removal columns on protein and peptide samples for mass spectrometric analysis. Poster presented as part of the 58th ASMS Conference on Mass Spectrometry and Allied Topics, May 23-27, 2010, Salt Lake City, Utah
Detergent Handbook & Selection Guide A Comprehensive Guide to the Properties and Uses of Detergents in Research. Also features information on detergent removal systems.
Highly efficient detergent removal for improved mass spectrometry analysis  Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionization when analyzed by mass spectrometry. Our DetergentOUT™ GBS10 resin removes free, unbound anionic, nonionic or zwitterionic detergents (e.g. SDS, Triton® X-100 or CHAPS) from aqueous protein and peptide samples with minimal sample loss for downstream analysis by mass spectrometry and other techniques.   Remove Free Aqueous Detergents Prevent Interference of Downstream Applications Improve Mass Spec. Detection The application note shows how DetergentOUT™ GBS10 improves mass spec detection of peptides after detergent removal.   Download the free application note to find the key to improved mass spectrometry analysis!
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Plant Proteomics Handbook
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Protein Purification Handbook
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment

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