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Brij® 35 (1 Citations)






Brij® 35

Brij® 35 is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution.

Proteomic Grade: DG003, DG004, DG503, DG515


Research Grade: 786-521, 786-351

 


Proteomic Grade Detergent Solutions

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds. The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl groups will form Schiff’s bases with the proteins that interfere with its structure.   

 



G-Biosciences’ Proteomic Grade Detergent Solutions contain reduced peroxides and carbonyl compounds. In addition, the detergents have less than 50µS conductivity. These detergents are offered as sterile, 10% aqueous solutions, sealed under inert gas, and are suitable for protein applications. These non-ionic detergents are suitable for isolating membrane-protein complexes.   

 


Other Proteomic Grade Detergent Solutions offered include: Triton® X-100, Triton® X-114Brij® 58Tween® 20Tween® 80 and Nonidet® P-40 Substitute. A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic grade detergents, as well as one gram samples of one of our Zwitterionic detergents (CHAPS) and one of our non-detergent sulfobetaines (NDSB 201).

 


Features

  • Full Name: Polyoxyethylene (23) lauryl ether
  • Synonym: Aldosperse L 9
  • CAS Number: 9002-92-0
  • Molecular Weight: 627
  • Absorbance (225nm): 0.07 (1% w/v)
  • Critical Micelle Concentration (CMC): 90µM
  • Aggregation Number: 24 to 40
  • Cloud Point: > 100º C
  • Average Micellar Weight: 48,000
  • Molecular Formula: C₁₀H₂₆O (OCHâ‚‚CHâ‚‚)₁₀

 

Applications

  • Non-ionic detergent available as a 10% proteomic grade solution or a research grade solid
  • For protein extraction, permeabilization of cells, and preparation of yeast spheroplasts
  • Ultra low concentrations of contaminating peroxides, aldehydes, salts and carbonyl compounds

 

 

Material Safety Data Sheet
786-351
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Finnish
Swedish
Polish
786-521
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
DG003
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Finnish
Swedish
Polish
DG004
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Finnish
Swedish
Polish
DG503C
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Finnish
Swedish
Polish
DG515C
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Detergent Handbook & Selection Guide A Comprehensive Guide to the Properties and Uses of Detergents in Research. Also features information on detergent removal systems.
Removing Detergents from Protein Solutions
Certificate Of Analysis
786-351
091006
100504
101506
102106
113011
121101
121804
132102
132410
132504
140602
142503
182401
182401-ex
191104
192307
192310
202901
DG003
082107
100906
110605
121412
122911
141203
161808
170302
DG004
062012
082107
100906
102911
DG503C
081108
091711
111808
121412
131208
131804
132506
140801
142608
143004
150302
151305
151607
152910
152910A
161706
161808
170302
171010
182201
182308
190901
190904
201706
DG515C
091711
100906
102911
110605
111808
113008
141203
143004
151305

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