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Brij® 58






Brij® 58

Brij® 58 is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution.

Proteomic Grade: DG005, DG006, DG505, DG516

Research Grade: 786-522, 786-352

 


Proteomic Grade Detergent Solutions

Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds. The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl groups will form Schiff’s bases with the proteins that interfere with its structure.   

 


G-Biosciences’ Proteomic Grade Detergent Solutions contain reduced peroxides and carbonyl compounds. In addition, the detergents have less than 50µS conductivity. These detergents are offered as sterile, 10% aqueous solutions, sealed under inert gas, and are suitable for protein applications. These non-ionic detergents are suitable for isolating membrane-protein complexes.   

 



Other Proteomic Grade Detergent Solutions offered include: Triton® X-100Triton® X-114Brij® 35Tween® 20Tween® 80 and Nonidet® P-40 Substitute. A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic grade detergents, as well as one gram samples of one of our Zwitterionic detergents (CHAPS) and one of our non-detergent sulfobetaines (NDSB 201).

 


Features

  • Synonym: Polyethylene glycol hexadecyl ether
  • CAS Number: 9004-95-9
  • Form: White solid (unless indicated)
  • Molecular Weight: 1122
  • Absorbance (225nm): 0.0788 (1% w/v)
  • Critical Micelle Concentration (CMC): 7-77µM
  • Aggregation Number: 70
  • Cloud Point: >100ºC
  • Average Micellar Weight: 79,000
  • Molecular Formula:  C16H33(OCH2CH2)20-OH

 


Applications

  • Non-ionic detergent available as a 10% proteomic grade solution or a research grade solid
  • For protein extraction, permeabilization of cells, and preparation of yeast spheroplasts
  • Ultra low concentrations of contaminating peroxides, aldehydes, salts and carbonyl compounds

 

 

Material Safety Data Sheet
786-352
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-522
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
DG005
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
DG006
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
DG505C
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
DG516C
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Detergent Handbook & Selection Guide A Comprehensive Guide to the Properties and Uses of Detergents in Research. Also features information on detergent removal systems.
Removing Detergents from Protein Solutions
Certificate Of Analysis
786-352
091009
101307
113011
130611
142805
181207-ex
190403
190403-ex
192309
192904
192904-ex
193112
200311
786-522
091512
101307
120401
120803
122401
DG005
191012
082306
101404
111506
122811
160806A
DG006
180208
191012
191012-arch
192005-arch
192706-arch
122811
DG505C
111506
122811
DG516C
100106
100801
122811
172906
180208
192005
192706

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