Fluorinated surfactants are unable to extract proteins from membrane, but are useful for membrane protein stabilization in subsequent purification steps as they do not strip natural lipids and other co-factors from the proteins. In addition, the bulky fluorinated tails can not penetrate into the interior and disrupt structure. Fluorinated surfactants often decrease non-specific aggregation and are thought to result in improved distribution on cryo-EM grids and better vitrification for cryo-EM data collection. They are also reported to increase crystallizability.
Specifications
- Compound Name: FTAC6
- IUPAC Name: S-(poly(tris(hydroxymethyl)acrylamidomethane)-(3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorothiooctyl) DPn=6
- Application: Membrane Protein Stabilization
- Surfactant type: Fluorinated surfactant
- Molecular Formula: n/a
- Molecular Weight: ~1400 g/mol
- Percent Composition: n/a
- Purity (HPLC, 214nm): n/d
- Retention time (RP18 HPLC): tR = 10.5 min
- Physical State: White powder
- Stability: Store in <-20°C freezer for up to one year
- Solubility: Soluble in water (18.5mM), methanol and DMSO
- Critical Micelle Concentration: 0.37mM