Fluorinated surfactants are unable to extract proteins from membrane, but are useful for membrane protein stabilization in subsequent purification steps as they do not strip natural lipids and other co-factors from the proteins. In addition, the bulky fluorinated tails can not penetrate into the interior and disrupt structure. Fluorinated surfactants often decrease non-specific aggregation and are thought to result in improved distribution on cryo-EM grids and better vitrification for cryo-EM data collection. They are also reported to increase crystallizability.
Specifications
- Compound Name: FTAC8
- IUPAC Name: S-(poly(tris(hydroxymethyl)acrylamidomethane)-(3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluorothiodecyl) DPn=12
- Application: Membrane Protein Stabilization
- Surfactant type: Fluorinated surfactant
- Molecular Formula: na
- Molecular Weight: ~2600 g/mol
- Percent Composition: na
- Purity (HPLC, 214nm): nd
- Retention time (RP18 HPLC): tR = 11.7 min
- Physical State: White powder
- Stability: Store in <-20°C freezer for up to one year
- Solubility: Soluble in water (1mM), methanol and DMSO
- Critical Micelle Concentration: 0.02 mM