ECH-Agarose

ECH-Agarose contains carboxyl functional group for coupling of affinity ligands via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting in 15-atom hydrophilic spacer arm. 

Amine groups of the ligands bind to the ECH-Agarose by carbodiimide coupling method. In this coupling method the free carboxyl group of the resin reacts with amine group of the ligand to form a stable peptide bond in the presence of water soluble carbodiimide such as EDC (N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide hydrochloride) at low pH (4-5). 

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Features:

• Resin Active group: -COOH groups
• Group to be coupled: -NH₂ groups  
• Resin Active group density: 50 µM carboxyl/ ml drained resin.
• 45-165µm particle size range
• Spherical, cross-linked agarose
• Resin spacer arm: 15 atom hydrophilic spacer arm.
• Coupling conditions: at 4°C- 25°C, pH: 4.5 -6 for 1.5 -24 hrs, Coupling could be done in organic solvents.
• No blocking reaction is required after the coupling reaction, saves time
• Storage: 2- 8°C

Application(s):

• ECH-Agarose contains carboxyl functional group for coupling of affinity ligands via their amine groups for affinity chromatography
• ECH-Agarose provides 15 atom hydrophilic spacer arm that enables coupling of small ligands containing amine groups