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EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.
The unstable nature of the intermediate in aqueous solutions makes 2-step coupling, however in conjunction with N-hydroxysuccinimide, a 2-step coupling is possible.
EDC is ideal for peptide immobilization and hapten-carrier protein conjugation.
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Features
Applications
Protocol | |
BC25-1 | ![]() |
BC25-5 | ![]() |
BC25-25 | ![]() |
BC25-50 | ![]() |
Material Safety Data Sheet | |
BC25-1 | |
BC25-5 | |
BC25-25 | |
BC25-50 |
Technical Literature | |
Biochemical Conjugation, Modification and Labeling of Oligonucleotide 5’ Phosphate Groups with EDC | Protocols and tips for coupling with the EDC crosslinker |
Protein Cross-Linking Handbook | A detailed guide to protein cross-linkers and their reactions. |
Certificate Of Analysis | |
BC25-5 | |
BC25-25 |