EDC
EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.
The unstable nature of the intermediate in aqueous solutions makes 2-step coupling, however in conjunction with N-hydroxysuccinimide, a 2-step coupling is possible.
EDC is ideal for peptide immobilization and hapten-carrier protein conjugation.
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Features
- EDC mediated coupling efficiency improved by N-hydroxysuccinimide
- Allows for 2-step coupling process
- Water soluble
- CAS Number: 25952-53-8
- Molecular Formula: C8H17N3•HCl
- Molecular Weight: 191.71 g/mol
- Spacer Arm (Å): 0
- Reactive Group: Carbodiimide
- Reactive Toward: Primary Amine + Carboxyl
- Membrane Permeable: NO
- Water Soluble: YES
- Cleavable/Reversible: NO
- Reaction pH: 4.7 to 6.0
Applications
- Couple haptens or peptides to carrier proteins
- Conjugate carboxyl to amine groups in peptides and proteins
- Cross link proteins to carboxyl surfaces and beads
- Convert carboxyls to amine-reactive Sulfo-NHS esters
- Activate nanoparticles to amine-reactive Sulfo-NHS esters
- DNA Labeling through 5' phosphate groups
| Protocol | |
| BC25-1 | |
| BC25-5 | |
| BC25-25 | |
| BC25-50 | |
| Material Safety Data Sheet | |
| BC25-1 | |
| BC25-5 | |
| BC25-25 | |
| BC25-50 | |
| Technical Literature | |
| Biochemical Conjugation, Modification and Labeling of Oligonucleotide 5’ Phosphate Groups with EDC | Protocols and tips for coupling with the EDC crosslinker |
| Protein Cross-Linking Handbook | A detailed guide to protein cross-linkers and their reactions. |
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- Gu, J. et al (2017) Development of antibody-modified chitosan nanoparticles for the targeted delivery of siRNA across the blood-brain barrier as a strategy for inhibiting HIV replication in astrocytes. Drug Deliv Transl Res.DOI: 10.1007/s13346-017-0368-5
- Beyzay, F et al (2017) Alpha Alumina Nanoparticle Conjugation to Cysteine Peptidase A and B: An Efficient Method for Autophagy Induction. AJMB. 9(2): 71
- Gu, J et al (2015) Mol Pharm. 0.1021/acs.molpharmaceut.5b00073
- Oncescu, V. et al (2014) PLOS. DOI: 10.1371/journal.pone.0089903
- Donkor, D.A. and Tang, X.S. (2014) Biomaterials. 35:3121
- Jiang, Q, et al (2013) J. Biomed. Mater. Res. A. 101A:1237
- Yang, S. et al (2013) Int. J. Nanomedicine. 8:2847
- He, P. et al (2013) Anal. Chim. Acta. 759:74
- Goddard, J.M. et al (2010) Colloid Surface B. 76:375
- Nugen, S.R. et al (2009) Biosens. Bioelectron. 24:2428
| Catalog | Description | Size | Price(USD) | Qty |
BC25-1 |
EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride) | 1g |
$20.00
|
|
BC25-5 |
EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride) | 5g |
$50.00
|
|
BC25-25 |
EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride) | 25g |
$172.00
|
|
BC25-50 |
EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride) | 50mg |
$24.00
|
