EDC

EDC

EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.

 

The unstable nature of the intermediate in aqueous solutions makes 2-step coupling, however in conjunction with N-hydroxysuccinimide, a 2-step coupling is possible.

 

EDC is ideal for peptide immobilization and hapten-carrier protein conjugation.

 

Click here to view our entire line of protein cross-linking and modification products.

 

Click here to see all of the cross-linking and modification accessories and tools available.

 

Click here to view our wide selection of protein cross-linkers.

 


Features

  • EDC mediated coupling efficiency improved by N-hydroxysuccinimide
  • Allows for 2-step coupling process
  • Water soluble
  • CAS Number: 25952-53-8
  • Molecular Formula: C8H17N3•HCl
  • Molecular Weight: 191.71 g/mol
  • Spacer Arm (Å): 0
  • Reactive Group: Carbodiimide
  • Reactive Toward: Primary Amine + Carboxyl
  • Membrane Permeable: NO
  • Water Soluble: YES
  • Cleavable/Reversible: NO
  • Reaction pH: 4.7 to 6.0

 


Applications

  • Couple haptens or peptides to carrier proteins
  • Conjugate carboxyl to amine groups in peptides and proteins 
  • Cross link proteins to carboxyl surfaces and beads
  • Convert carboxyls to amine-reactive Sulfo-NHS esters
  • Activate nanoparticles to amine-reactive Sulfo-NHS esters
  • DNA Labeling through 5' phosphate groups

 

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Protocol
BC25-1
BC25-5
BC25-25
BC25-50
Material Safety Data Sheet
BC25-1
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BC25-5
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BC25-25
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BC25-50
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Technical Literature
Biochemical Conjugation, Modification and Labeling of Oligonucleotide 5’ Phosphate Groups with EDC Protocols and tips for coupling with the EDC crosslinker
Protein Cross-Linking Handbook  A detailed guide to protein cross-linkers and their reactions.
Catalog Description Size Price(USD) Qty
BC25-1
EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride) 1g
$20.00
BC25-5
EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride) 5g
$50.00
BC25-25
EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride) 25g
$172.00
BC25-50
EDC (1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride) 50mg
$24.00