Using a Miniprep Kit for pAmylase Plasmid Isolation (Lab 8G)

Using a Miniprep Kit for pAmylase Plasmid Isolation (Lab 8G)

This kit is designed to teach 8 groups of four students the principles of plasmid DNA purification by minipreps.

 The kit contains the reagents to allow students to:

  • Collect pAmylase-transformed bacteria from an overnight culture
  • Resuspend bacteria, lyse and isolate the pAmylase plasmid by removing proteins and genomic DNA
  • Bind the plasmid DNA to a spin column and wash
  • Elute the plasmid DNA from column
  • Measure the amount of purified pAmylase plasmid DNA with a unique DNA assay

 



Features

  • For 8 groups of 4 students 
  • Requires high speed micro-centrifuge, micropipettes and tips


Applications

  • HS-LS1: From Molecules to Organisms: Structures and Processes
  • LS1.A: Structure and Function

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PROTOCOL
BTNM-8G
SDS
BTNM-8G
Catalog Description Size Price(USD) Qty
BTNM-8G
The rAmylase Project: Using A Miniprep Kit for Plasmid Isolation 8 x 4 student groups
$127.00