Biotechnology, Science for the New Millennium

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G-Biosciences is the preferred vendor for Ellyn Daugherty's Biotechnology, Science for the New Millennium Program.

Biotechnology: Science for the New Millennium is a comprehensive program that prepares adults and teenagers for a variety of post-secondary options including community or career college biotechnology certificate programs, four-year biotechnology degree programs, and industry workplaces. The vast text and laboratory curriculum was development and improved with input from an industry advisory committee composed of laboratory internship mentors. Biotechnology: Science for the New Millennium allows students to increase conceptual background as they build lab proficiencies. Science-bound and business-bound students benefit from the extensive skill development, science methods, and career exploration that permeate the curriculum.

 

Biotechnology: Science for the New Milennium (BS4NM): The rAmylase Project Kits

  1. Characterizing Proteins by PAGE
  2. Assaying for Amylase Activity
  3. Direct ELISA of Bacterial α-Amylase
  4. Western Blot to Identify α-Amylase
  5. Determining the Concentration of Amylase in a Solution
  6. Restriction Digestion to Verify the pAmylase Plasmid
  7. Transformation of E. coli with pAmylase
  8. Using A Miniprep Kit for Plasmid Isolation
  9. Using Ion Exchange Chromatography to Separate Proteins
  10. Confirming the Amylase Gene Using PCR

Other Biotechnology: Science for the New Milennium (BS4NM) Kits

  1. Bacterial Gram Staining (Lab 4L)
  2. Genomic DNA Isolation (Lab 4H)

Biotechnology Basics™ by Ellyn Daugherty

  1. Cheese Biotechnology: Cheese Production: The Evolution of Cheese-Making Technology (Lab 1C)
  2. DNA Spooling with a Twist: Genomic DNA Spooling (Lab 4B)
  3. Micropipetting Mastery: Micropipetting: Measuring Small Volumes in Biotechnology (Lab 3B)
  4. Gel Box Science
  5. Crime Scene DNA Analysis using RFLP Technology

  

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Micropipetting: Measuring Very Small Volumes in Biotechnology

This Biotechnology Basics™ by Ellyn Daugherty is designed to teach students the basic principles of measuring small volumes (<500µl) using micropipettes. The kit is supplied with all th..

pAmylase2014

Amylase producing plasmid for Ellyn Daugherty Biotechnology:Science for the New Millennium course. See the figure for key restriction and structural elelments of the plasmid. ..

Restriction Digestion to Verify the p-Amylase Plasmid (Lab 8b)

This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to: Set up restrictio..

Transformation of E. coli with p-Amylase (Lab 8C)

This kit is designed to teach 8 groups of four students the principles of bacterial transformation.   The kit contains the reagents to allow students to: Generate E.coli comptent cell..

Using a Miniprep Kit for p-Amylase Plasmid Isolation (Lab 8G)

This kit is designed to teach 8 groups of four students the principles of plasmid DNA purification by minipreps.   The kit contains the reagents to allow students to: Collect p-Amylas..

Using Ion Exchange Chromatography to Separate Proteins (Lab 9C)

This kit is designed to teach 8 groups of four students the principles of Ion Exchange Chromatography and protein purification.  The kit contains the reagents to allow students to: Equilibr..

Western Blot to Identify Alpha-Amylase (Lab 6G)

This kit is designed to teach 8 groups of four students the principles of Western blotting.   The kit contains the reagents to allow students to: Conduct a serial dilution of a stock ..