This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach students about RFLP (restriction fragment length polymorphisms) and its use in crime scene investigations. Students will use restriction enzyme digestions of victim, suspect and crime scene DNA and then visualize the digested fragments using horizontal electrophoresis. Students will practice loading samples and then load the restriction digests and visualize the DNA fragments using G-Biosciences LabSafe™ Nucleic Acid Stain.
- Understand RFLP (restriction fragment length polymorphisms)
- Perform restriction digestions
- Perform horizontal agarose gel electrophoresis
- Visualize and compare DNA fragments
- Conduct pseudo crime scene investigation
This laboratory activity is adapted from labs in Biotechnology: Science for the New Millennium by Ellyn Daugherty and G-Biosciences’ DNA Fingerprinting Lab (BE-104). For more information about Biotechnology: Laboratory Manual curriculum, please visit www.emcp.com/biotechnology. This kit is produced under license from Paradigm Publishing, Inc., a division of New Mountain Learning.