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Immunoaffinity Chromatography

A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilized on a chromatographic resin.

 

The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction.

 

In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilized in research laboratories.

 

Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.

 


Features

  • Teaches antibody:antigen precipitation.
  • Hands-on activity to precipitate antigen and antibody complex.
  • Quantitative assessment of antibody concentration.

 

 

Protocol
BE-507
Material Safety Data Sheet
BE-507
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Technical Literature
Life Science Educational Program  A guide to the educational training products offered by G-Biosciences

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