Immunoprecipitation is a routinely used technique that removes a protein or peptide, which specifically reacts with an antibody, from a solution. The name of the technique is a misnomer as the interaction of the peptide or protein with the antibody does not cause precipitation. The “precipitation” is caused by an immunoglobulin binding protein, such as protein A or protein G, immobilized to a solid support or bead. The protein A or G binds the antibody-antigen complex and the complex is precipitated and removed from the solution by spinning down the beads.
This kit contains all the reagents necessary to immunoprecipitate a specific protein from a complex sample.
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
Supplied with needed reagents and supplies.
- Hands-on activity of immunoprecipitation and isolate specific antigen and antibody.
- Teaches immunoprecipitation technique.
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