Western Blot Analysis (4 Citations)
Lab activity that teaches use of specific lock-key matching properties of antigen-antibody for detection and characterization of antibody or antigenic proteins in complex samples. One such widely used method is known as Western Blot analysis. Western Blot analysis involves electrophoresis separation of test protein samples on polyacrylamide gels, then the separated protein samples are electroblotted on a protein binding membrane – where protein is captured and immobilized. The membrane containing transferred proteins is probed with a specific antibody and a matching detection probe.
In this lab activity, students carry out Western Blot procedure and screen test samples from a population of infected individuals and group them according to the precise nature of their disease. Students learn how this technique is applied in the real world, such as detection and characterization of specific marker proteins in clinical testing laboratories.
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
- Supplied with necessary reagents and supplies for a complete analysis
- Supplied with simulated test samples
- Teaches Western Blot analysis technique.
- Learn to perform Western Blot analysis and screen unknown sample, technique used by many research and testing laboratories.