Science Education
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A comprehensive program that prepares adults and teenagers for a variety of post-secondary options including community or career college biotechnology certificate programs, four-year biotechnology degree programs, and industry workplaces. The vast text and laboratory curriculum was development and improved with input from an industry advisory committee composed of laboratory internship mentors. Biotechnology: Science for the New Millennium allows students to increase conceptual background as they build lab proficiencies. Science-bound and business-bound students benefit from the extensive skill development, science methods, and career exploration that permeate the curriculum.
BioScience Excellence™ Contents
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The BioScience Excellence™ program contents have been divided into six distinct sections. Each section consists of several teaching modules with information provided as to how these modules are connected, and their appropriate order for training purposes. These sections collectively cover the length and breadth of modern life science topics and are adequate for achieving the highest standard for biotechnology education of the most rigorous school, college, and university training worldwide.
The General Biotechnology topic deals with the very basic components of life sciences and gives an overview of the scope of biotechnology. This section is suitable for conveying an understanding of genes, proteins, and their interactions.
The Microbial Studies covers handling of microorganisms, an important tool in biotechnology.
The Molecular Biology and Genomic topics cover the multitudes of molecular cloning and recombination techniques.
The Protein and Proteomic section covers various aspects of protein molecules, including composition, structure, function, and various analytical and related techniques.
The Immunotechnology Studies deals with sophisticated immunotechnology detection techniques.
The Miscellaneous Biotechniques section includes various widely used laboratory techniques in protein and nucleic acid research.
Curiosity Based Life Sciences Educational Programs
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Biotechnology has been described by various commentators as one of the most important technologies of the 21st century. Biotechnology promises to have the potential to solve a wide variety of challenges faced by modern society, including the supply of quality food materials, pharmaceuticals, healthcare, forensic science, environmental concerns and security needs.
The emergence of biotechnology has created a world demand for trained scientists, technicians, and a variety of allied professionals. Hence, teaching topics in biotechnology are now standard in educational programs worldwide.
Recognizing the significance and challenges of life sciences education, G-Biosciences has initiated the BioScience Excellence™ program. The program features hands-on teaching kits based on inquiry and curiosity that explore the fundamentals of life sciences and relate the techniques to the real world around us. The BioScience Excellence™ teaching tools will capture the imagination of young minds and deepen their understanding of various principles and techniques in biotechnology and improve their understanding of various social and ethical issues.
International Standard for Biotechnology Education
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The BioScience Excellence™ program has been developed to meet the demands of teaching life sciences. Scientists, professionals, and teachers from the USA, Europe, and Asia have developed the BioScience Excellence™ program. The aim of this BioScience Excellence™ program is to provide a training program of international standard that will prepare students to compete in a global economy driven by biotechnology. The curricula, teaching tools, and kits have been designed to meet the expectations of the most rigorous school and college training worldwide.
The BioScience Excellence™ program offers hands-on and curiosity based laboratory activities to deepen understanding on selected subjects, such as genes, proteins and their interaction within cells. The program enables the learning of biotechnology by posing questions, exploring and utilizing the real world, hands-on exploration that stimulates the interest of students and makes the process of learning interesting.
Biotechnology Careers
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A major aim of the BioScience Excellence™ program is to prepare students for the vast career possibilities in the life sciences field, such as pharmaceutical development, agriculture, genetic engineering, environmental sciences, forensics, ecology, evolution, and education. Comprehensive training based on the BioScience Excellence™ program will deepen understanding of both genomics and proteomics. More importantly, the program builds students' confidence for their next career challenges, be that further education or seeking employment in the ever-growing biotechnology industry.
Learning by Hands-on Activity and Curious Exploration
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The key strategy of the BioScience Excellence™ program is to pose questions and to challenge students to think, explore, and understand how various processes in life sciences work and their application to the real world, as well as their social implications. Our objective has been to design and develop kits for active participation and problem solving.
Given the rapid growth of information and techniques, the kits are designed to give the most up to date foundation in current technical concepts and scientific methods. Each kit consists of three main components; materials for hands-on activities, “Teacher’s Guide”, and a “Student’s Guide”. The “Teacher’s Guide” provides detailed principles behind the techniques to be introduced, and where feasible, a real world scenario to increase interest in the techniques and step-by-step instructions on conducting each laboratory activity. The “Student’s Guide” details a step-by-step procedure for the laboratory activity, lab activity record sheets, and teaches how to analyze results and draw conclusions.
The kits will challenge students to think, participate in hands-on activities, collect data, analyze and interpret findings, and draw conclusions based on the findings and observations. And finally students learn how to relate and employ what is learned in the classroom to the real world of science – a skill that is a hallmark of quality education in industrialized nations.
BioScience Excellence™ Kits for All Levels of Learning
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Depending on the concept and depth of information needed to be taught, the BioScience Excellence™ program offers kits that can be easily adapted. The range of kits offered under the BioScience Excellence™ program is suitable for training students from school through university levels.
The BioScience Excellence™ program can also be adapted for professional development programs for teachers, technicians, and other professionals with little or no formal science training.
Support and Services
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G-Biosciences is committed to educational excellence and our aim is to help provide support and services beyond teaching kits. G-Biosciences has extensive experience in cutting edge research in life sciences and offers a wide variety of fine biochemicals, research tools, kits, and devices. G-Biosciences will help you acquire tools, resources, and supplies that will enable you to conduct modern educational and instructional programs. G-Biosciences also offers help to teachers and institutions in the development of curriculum and setting up modern laboratories.
Affinity chromatography is a powerful tool for the purification of specific biomolecules, including proteins. The basic principle is that a biospecific ligand is immobilized to a solid support or resin to which a solution containing the protein of interest is passed over. Ligands are often based on ..
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The following supplies are used in agarose gel electrophoresis. Products include reagents for preparation of gels, running agarose gels and staining gels.
Agarose: A low melt agarose for casting gels. Supplied in a convenient 50gm size.
TAE Buffer: A concentrated 50 times (..
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Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistan..
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Gene/Protein:
This antibody lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how highly specific antigen and antibody interactions can be exploited as a tool for research and analysis. This antigen-antibody study involves the use of an immunodiffusion techniqu..
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Gene/Protein:
Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics.
The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches st..
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Gene/Protein:
This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates.
This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary, and decline o..
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Gene/Protein:
LB Broth & LB Agar
The bacterial growth media available are LB (Luria and Bertani); both as liquid and solid media for making solid growth matrices and bacterial plates, such as Petri Dishes. The LB media supplies all the nutrients required for bacterial growth.
LB Broth is for..
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Bacterial transformation allows researchers to insert their recombinant DNA into bacteria, which then multiply making more copies of the transformed bacteria. The transformed plasmid can also be used by the bacteria as a template to make recombinant protein.
Students will explore the..
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Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalog plant leaf samples from a diverse group of locally available plants. This lab activ..
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The subjects of life sciences and biotechnology allow students to learn about the fundamental building blocks of life, including their proteins. In addition, biotechnology teaches us how scientists manipulate proteins in industrial applications.
This kit allows students to study their own p..
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Gene/Protein:
Our Bacterial Gram Staining kit is supplied with three strains of bacteria and all the necessary prealiquoted components to carry out the Gram staining to determine Gram positive and negative bacteria.
The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christia..
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This kit is designed to teach 8 groups of four students the principles of Protein Electrophoresis.
The kit contains the reagents to allow students to:
Conduct a serial dilution of a stock amylase solution
Prepare samples for PAGE electrophoresis
Separate and distinguish proteins by electrophor..
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Gene/Protein:
This kit is designed to teach 8 groups of four students the principles of cheese making.
The cheese making technology kit contains the reagents to allow students to:
Set up a controlled experiment & collect numerical data
Determine which curdling agent tested produces cheese..
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Gene/Protein:
This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)
The kit contains the reagents to allow students to:
Set up multiple PCR reactions using pAmylase plasmid DNA and bacterial genomic DNA templates
Set up a PCR negative co..
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The protein make-up of an organism is unique to that individual species, however many essential proteins are highly conserved. This level of conservation can be seen when protein fingerprinting is used to compare and contrast different organisms. The Conservation of Genetic Information ki..
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Gene/Protein:
This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach students about RFLP (restriction fragment length polymorphisms) and its use in crime scene investigations. Students will use restriction enzyme digestions of victim, suspect and crime scene DNA and then visualize the digest..
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This kit is designed to teach 8 groups of four students the principles of protein concentration estimation assays. The kit contains the reagents to allow students to:
Conduct a serial dilution of a stock amylase solution to create standards of known concentration
Use a Bradford protein assay to ..
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Gene/Protein:
Dialysis is a routinely used technique in research laboratories to “change” the solution a biomolecule is dissolved in. Often the buffers used to isolate biomolecules, such as proteins, are not compatible with downstream applications due to high concentrations of reagents, such as s..
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This kit is designed to teach 8 groups of four students the principles of ELISA (Enzyme Linked ImmunoSorbent Assays).
The kit contains the reagents to allow students to:
Conduct a serial dilution of a stock amylase solution to create standards of known concentration
Bind known and unkno..
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Gene/Protein:
Today’s media routinely covers forensic stories of criminals captured and innocents released using the powerful DNA fingerprinting technique. This kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a pseudo-cri..
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Gene/Protein:
Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyzes the formation of phosphodiester bonds.
..
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Restriction enzymes are DNA-cutting enzymes found in bacteria and as they cut within the molecule, are often called restriction endonucleases. A restriction enzyme recognizes and cuts DNA only at a particular unique sequence of nucleotides, allowing for restriction sites to be mapped.
The D..
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One of the most important molecules in an organism is its genomic DNA. Genomic DNA is found in every cell of an organism and carries all the necessary information required to make that organism.
This kit provides all the reagents required to visualize genomic DNA strands by a process ..
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Gene/Protein:
This lab activity teaches the use of highly specific matching properties of antigen-antibody for detection of antigenic or antibody proteins in complex samples.
One such immunodetection method is widely known as Dot Blot. The method involves applying small volumes of antigens or test sa..
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Nucleic acids (DNA and RNA) and proteins are routinely resolved by electrophoresis, which separates molecules based on size and/or charge. Following electrophoresis, the molecules need to be extracted from the electrophoresis medium for use in downstream applications and experiments. Electroelu..
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Gene/Protein:
Enzymic proteins are functional molecular engines of life and as such carry out cellular reactions in cells. Enzymes convert a target molecule (substrate) into a different molecule (product). Enzymes have a number of distinct advantages over conventional chemical catalysts. The most distinctive feat..
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The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein.
Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, in..
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Membranes play a crucial role in cells by enclosing specific compartments and regulating the entry and exit of metabolites and other crucial components in and out of a cell. They also play a key role in signaling from plasma membrane receptors to the cell nucleus.
The key ..
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Gene/Protein:
This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach the basic principles of a gel box, specifically horizontal agarose gel electrophoresis. The kit is supplied with all the reagents necessary to conduct 4 gel box science experiments, each using a gel box (not supplied).
T..
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A major goal of genetic engineering is to have the ability to correct genetic defects to treat genetic diseases, such as cystic fibrosis, sickle cell anemia and Huntington’s disease.
One technique to correct genetic defects in bacteria is to introduce the corrected gene back int..
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Our Genomic DNA Isolation kits is supplied with dried E.coli and all the ncessary prealiquoted components to isolate bacterial genomic DNA from the bacteria and use G-Biosicences Nucleic Acid Assay to determine the amount of DNA extracted. In addition, a class demonstration is include to demonstrate..
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Gene/Protein:
Our Genomic DNA Spooling kit allows for 8 groups of 2-4 students to spool salmon testes DNA onto a spooling rod. This lab module allows students to precipitate the DNA from solution before spooling (winding) onto a rod. The recovered DNA is returned to solution and the students then use ..
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Gene/Protein:
Can E. coli cells be transformed into cells that have two new characteristics (ampicillin-resistance and green fluorescent protein production)? This Biotechnology Basics™ kit by Ellyn Daugherty is designed to teach students the basic principles of plasmids, antibiotic resistance, competen..
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Water pollution is a major problem throughout the world, raising severe concerns about health and sanitization issues. A common problem in polluted water is the presence of bacteria, particularly coliform bacteria from fecal matter, which can directly result in disease, as well as act as a mar..
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Recent proteomic studies have led scientists to estimate that there are almost a million different proteins in a single human cell. The function and properties of these proteins are highly distinct ranging from structural proteins involved in cell integrity, including hydrophobic cell membrane..
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Hydrophobic chromatography is based on the fact that protein molecules can have extensive hydrophobic regions. These hydrophobic regions, in media favoring hydrophobic interactions, such as an aqueous solution with high salt concentration, can bind to hydrophobic ligands coupled to an uncharge..
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Gene/Protein:
A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilized on a chromatographic resin.
The solution cont..
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Immunoprecipitation is a routinely used technique that removes a protein or peptide, which specifically reacts with an antibody, from a solution. The name of the technique is a misnomer as the interaction of the peptide or protein with the antibody does not cause precipitation. The “precipitat..
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-500x500h.jpg "Immunoquantification Technique (ELISA)")
Gene/Protein:
Lab activity that teaches the use of lock-key matching properties of antigen-antibody for detection as well as quantification of antibody or antigenic proteins in complex samples. This method is widely known as Enzyme Linked Immunosorbent Assay, or ELISA.
In this lab activity, students ..
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Gene/Protein:
The following section includes the reagents used in the Immunotechnology section.
BE Antibody 4 (HRP Secondary): A goat anti-rabbit IgG secondary antibody labeled with the horseradish peroxidase (HRP) enzyme.
HRP Substrate: A colorimetric substrate for detecting t..
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Agarose gel electrophoresis is a routinely used tool for separating nucleic acids.
Nucleic acids are negatively charged molecules, which when loaded onto the solid agarose matrix, migrate in the presence of an electric field, separating the nucleic acids by size.
This kit pro..
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Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobi..
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Gene/Protein:
Genomic DNA isolation is a crucial technique in molecular biology, genetic manipulation and biotechnology. To carry out these exciting and innovative sciences, researchers require an original source of DNA, which is easy to acquire as it is found in every living organism. Genomic DNA is ..
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An interesting hands-on lab activity that teaches students the skills required for the isolation of bacteria from test samples. This kit teaches aseptic handling techniques and cultivation of bacteria.
Using bacterial culture techniques, students discover and isolate the bacteria present in..
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Microorganisms, such as bacteria and fungi, are all around us. This kit allows students to collect samples from the world around them and grow the microorganisms so that students can visualize the microorganisms. This teaches students how to plate and grow microorganisms.
Supplied wit..
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Gene/Protein:
This Biotechnology Basics™ by Ellyn Daugherty is designed to teach students the basic principles of measuring small volumes (<500µl) using micropipettes. The kit is supplied with all the reagents to allow 8 groups of 4 students to learn about P-1000, P-200 or P-100 and P-20 or P-10 mi..
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A PCR Based Laboratory Research Experiment. A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental.
Students l..
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The key biotechnology molecule that has probably had the most media coverage is DNA (also referred to as genes and genomes) that supplies the blueprint for each individual. To grip your students imagination and introduce them to DNA have them see their very own genome. This exercise allo..
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The kit utilizes the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.
Nucleic acid concentration can be measured by measuring the diameter of diff used nucleic acid spots or comparing the color density of the spots..
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Gene/Protein:
Isolation of genomic DNA is an essential technique in modern research science, particularly molecular biology and biotechnology.
Genomic DNA is purified from a multitude of sources including mammalian tissue, such as cheek cells (BE-303), plant cells or bacterial cells.
These kits use de..
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Amylase producing plasmid for Ellyn Daugherty Biotechnology:Science for the New Millennium course.
See the figure for key restriction and structural elelments of the plasmid...
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Proteins are the building blocks of life and there are estimated to be almost 1 million different proteins in a normal animal cell. Each protein has very different and unique physical properties. The Physical Properties of Proteins kit is a lab activity that enables students to investigate the..
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Gene/Protein:
Bacterial plasmids, the non-genomic transferable DNA, can easily be purified from bacteria using numerous techniques. The purification of DNA is important for genetic research as it provides a source of transferable DNA and allows researchers to isolate large amounts of recombinant DNA.
&nb..
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Bacterial plasmids, the non-genomic transferable DNA, can easily be purified from bacteria using numerous techniques. The purification of DNA is important for genetic research as it provides a source of transferable DNA and allows researchers to isolate large amounts of recombinant DNA.
..
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A major factor in the advancement of the genomic field and subsequent sequencing of the human genome was the ability to amplify the amount of genes, giving researchers an unlimited supply of DNA. This technique, which has also been a major contributor to the advancement of molecular biology, i..
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Gene/Protein:
The Polymerase Chain Reaction, or PCR, is routinely used for teaching amplification of tiny amounts of DNA, or genes. PCR grade reagents are offered in stabilized or dry forms for greater stability and convenience of use in educational setting:
Taq DNA polymerase: A thermostable D..
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Gene/Protein:
A single protein molecule rarely carries out its role in the body on its own. Many proteins and cofactors are often involved and act as regulators, activators or inhibitors of the protein’s function. Researchers are constantly on the hunt for biomolecules that interact with their pro..
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The cellular environment is a constant dynamic with the continued synthesis, degradation and recycling of protein molecules. This dynamic allows for close regulation of cellular processes by switching off cellular pathways by degrading key proteins. The degradation of proteins is controlled an..
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An important and essential tool in protein analysis is electrophoresis. Protein electrophoresis allows researchers to separate complex protein samples by their molecular size and/or net charge on polyacrylamide gels. The proteins can be subsequently visualized with various protein stains..
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Polyacrylamide gel electrophoresis (PAGE) is regularly used in protein research to separate a mixture of proteins by their molecular weight. Protein electrophoresis uses polyacrylamide gels that are made, or cast, from a mixture of acrylamide and bisacrylamide that is cross-linked by the actio..
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Each tissue of an organism performs a specific function, for example, the heart has a completely different role than that of the brain. The diverse functions of an organism’s tissues is due to the protein make-up, or fingerprint, of the tissue. Each organ contains specialized prote..
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A proteins life cycle begins once its gene is turned on and its mRNA is transcribed and translated to produce the polypeptide strand. The correct folding of a protein is the first crucial stage of the life cycle of a protein. Correct folding is essential for a protein to be functional, whereas incor..
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The aim of proteomics is to identify and characterize all the proteins in an organism. The major restrictions in protein identification are the large number of proteins and the huge differences in abundance. These restrictions mean low abundance, critical proteins are often masked and ar..
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Gene/Protein:
Protein labeling is an important technique that allows researchers to study a protein’s location, movement and interactions within a living cell. The most common labels used are fluorescent labels that allow researchers to study proteins with fluorescent microscopy techniques. Fluor..
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Prepacked purification columns for purification are available in packs of 5. The columns available are the anionic chromatography columns, hydrophobic columns, size exclusion (gel filtration) columns...
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Proteomics often involves the purification and identification of novel proteins from tissues. The strategy often employed is one using multiple and different protein purification techniques to fractionate proteins into specific fractions. This allows for easier identification and charact..
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This lab activity has two objectives, on one hand it is designed to deepen the understanding of protein molecules. On the other hand, students also learn the potential of electrophoresis in protein analysis.
Students study the fundamentals of protein structure from their primary structure ..
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Agarose electrophoresis is an important tool for isolating DNA by its molecular weight; following separation of DNA molecules, the DNA needs to be purified from the agarose for further applications. The Purification of a Gene kit teaches the recovery and purification of DNA fragments follow..
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The Quantitative Precipitin Technique is a simple technique that is routinely used in the analysis of antibody and antigen interactions and for the estimation of the antibody or antigen content in a sample. The technique is based on the interaction of antibody and antigen to form a large protein com..
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Molecular cloning is a commonly used technique for the generation of recombinant proteins. The gene for a protein of interest is cloned into a vector, or plasmid, in frame with the gene for a protein tag. These tags are used to purify expressed protein by established methodologies. ..
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This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to:
Set up restriction digests of the p-Amylase plasmid with two enzymes
Set up positive and negative controls for the rest..
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Gene/Protein:
RNA isolation is an important tool in understanding gene and protein expression regulation. The RNA Isolation kit is a safe RNA isolation kit as it does not utilize toxic phenol or chloroform. Each kit is provided with an extraction buffer for the lysis of biological samples and inhibition of RNase,..
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Size exclusion chromatography (SEC), also called gel filtration chromatography or gel-permeation chromatography (GPC), uses porous particles to separate molecules of different sizes. It is generally used to separate biological molecules and to determine molecular weights and molecular weight d..
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Southern blot analysis, named after its inventor Edwin M. Southern, is a common research technique for enhancing the results of agarose electrophoresis. DNA is resolved on an agarose gel and is subsequently transferred to a nylon membrane.
The resulting membrane can be probed for spec..
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