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Tissue PE LB™

Catalog Description Size Price(USD) Qty
786-181
PE LB™ for Tissue 500ml
$115.00
786-181T
PE LB™ for Tissue 50ml
$28.00

Tissue PE LB™

Tissue-PE LB™ has been developed for extraction of total soluble protein from animal tissues.  Tissue- PE LB™ is based on an organic buffer, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.  Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue-PE LB™. Tissue- PE LB™ reagent has been tested for use with a wide variety of animal tissues, fresh as well as frozen tissues. 

 

The protein extract prepared with Tissue-PE LB™ may be used for most enzyme assays including reporter gene assays (e.g. β-galactosidase, luciferace, chloramphenical acetytransferease), kinases (e.g. PKC, PKA, Tyrosin Kinase), and immunoassays (e.g., ELISA, Western blots, RIA).

 

COMPATIBILITY

Tissue-PE LB™ is compatible with most downstream applications including various enzyme assays, running various chromatography, and gel electrophoresis applications. Tissue-PE LB™ is also compatible for protein estimation with NI™ (Non-Interfering) Protein Assay.

 


Features

  • Provides a simple and versatile method for protein extraction from fresh or frozen tissues.
  • Compatible with most enzyme assays including:
    • Reporter gene assays; ß-galactosidase, luciferase, chloramphenicol acetyltransferase.
    • Kinases; Protein kinase C, protein kinase A, tyrosine kinase.
    • Immunoassays; ELISA, Western blots, RIA.
  • Compatible with electrophoresis and chromatographic applications.

 


Applications

  • For extraction of soluble proteins from fresh and frozen animal tissue.
  • Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis.
     

 

 

Protocol
786-181
786-181T
Material Safety Data Sheet
786-181
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786-181T
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Technical Literature
Isolate Biologically Active Proteins Our range of Protein Extraction & Lysis Buffer (PE LB™) systems isolate biologically active proteins from bacteria, yeast, mammalian cells and tissue and insect cells.
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
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