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Yeast PE LB™

Catalog Description Size Price(USD) Qty
786-178
PE LB™ for Yeast with Zymolyase® 100 Preps
$166.00
786-179
PE LB™ for Yeast 500ml
$115.00

Yeast PE LB™

Yeast PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells. This kit is provided with an optional protocol to make spheroplast and remove lytic enzyme Zymolyase®, prior to lysis and extraction of yeast proteins. Yeast PE LB™ is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided.  Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast- PE LB™ (see Related Products for protease inhibitor Protease Arrest™). The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast PE LB™ eliminates the need for laborious glass bead lysis of yeast cells. The kit is good for 100 preps of 50µl yeast cell pellet.

 

APPLICATIONS

Preparation of yeast spheroplast and extraction of yeast proteins. This kit is suitable for processing approximately 10ml yeast cell pellet suspension, either single or multiple smaller preps. 

 

COMPATIBILITY

Yeast PE LB™ is compatible with any downstream application including running various chromatography procedures and gel electrophoresis applications. Yeast PE LB™ is also compatible for protein estimation with NI™ protein assay

 


Features

  • Eliminates the need for glass bead lysis.
  • Supplied as a kit, containing Zymolyase® and reagents needed for protein extraction.
  • Suitable for processing 100 x 50µl yeast cell pellet preparations.

 



Applications

  • Lysis and extraction of proteins from yeast cells.
  • Isolation of spheroplasts.
  • For the isolation of biologically active proteins.
  • Suitable for most applications including enzyme and protein purifications, electrophoresis, Western blotting and 2D-gel analysis.

 

 

Protocol
786-178
786-179
Material Safety Data Sheet
786-178
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786-179
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Technical Literature
Isolate Biologically Active Proteins Our range of Protein Extraction & Lysis Buffer (PE LB™) systems isolate biologically active proteins from bacteria, yeast, mammalian cells and tissue and insect cells.
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
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