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Extraction and Lysis Systems

Download the Protein Sample Preparation Handbook

 

We offer a wide variety of products for protein extraction and lysis.

 

Our protein extraction and lysis buffer systems (PE LB™) ensure good protein recovery, while maintaining the biological activity of the proteins. The solubilized proteins are suitable for enzyme assays, electrophoresis, folding studies, chromatographic studies and many other downstream applications. The PE LB™ systems offer a wide selection of buffers for lysis and extraction of proteins from bacteria, yeast, mammalian cell cultures and tissues.

 

FOCUS™ Proteome Kits are for the preparation of total protein, including soluble, insoluble, membrane, cytoplasmic, nuclear, signal, phospho- and glyco- proteins. These kits are simple to use, save time, improve the quality of protein analysis and enhance the chances of discovery of novel proteins.  They are suitable for the analysis of proteins using electrophoresis and other biochemical techniques and are compatible with biological samples from tissues, cells, plants, yeast, bacteria, insects and biological samples.

 

Our lysis buffers and kits include RIPA lysis buffer, a complete lysis buffer; an inclusion bodies solubilzation buffer, IBS™ Buffer; kits for total protein extraction (TPE™) for the extraction of total proteins from cells and tissues, and an RBC lysis buffer specifically designed for the lysis of red blood cells.

 

Accessories are available to assist with protein extraction and isolation procedures which include our easy grinding resin, a highly efficient grinding resin with matching pestles and Molecular Grinding Resin™ which is available with matching pestles and tubes. We also offer ProteaseArrest™, a general and complete protease inhibitor cocktail solution.

Insect Cell-PE LB™ has been developed for extraction of cytoplasmic soluble protein from insect cultured cells. The Insect Cell-PE LB™ is based on organic buffering agents, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance ext..
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A Grinding Resin For Proteomics & Molecular Biology Applications Molecular Grinding Resin™ has been specifically developed for effectively grinding small biological samples for the preparation and extraction of DNA, RNA and proteins. The resin consists of high tensile micro-particles, whi..
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Tissue-PE LB™ has been developed for extraction of total soluble protein from animal tissues.  Tissue- PE LB™ is based on an organic buffer, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance extraction and stability of pr..
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The TPE™ kit is a universal lysis system designed for the solubilization and extraction of total proteins from cells and tissues for SDS-PAGE Analysis.  The kit is suitable for animal, plant, yeast, bacteria, and other biological samples.   The TPE™ kit provides a two compo..
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Yeast PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells. This kit is provided with an optional protocol to make spheroplas..
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Model:
Mammalian Cell PE LB™ has been developed for extraction of total soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance..
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Model:
A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techni..
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