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FOCUS™ ProteaseArrest™ Protease Inhibitor Cocktail

Protease Inhibitor Cocktail
Catalog Description Size Price(USD) Qty
786-108F
FOCUS™ ProteaseArrest™ [100X] 1ml
$168.00

FOCUS™ ProteaseArrest™ Protease Inhibitor Cocktail

FOCUS™ ProteaseArrest™ is a ready-to-use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry. The protease inhibitor cocktail consits of AEBSF, Aprotinin, Bestatin, Calpain Inhibitor I, Leupeptin, Phosphoramidon and PMSF that inhibits serine, cysteine, calpain and metallo- proteases. Due to the optimized concentration of the various inhibitors, the FOCUS™ ProteaseArrest™ shows excellent inhibition of protease activities, and is therefore suitable for the protection of protein samples from animal tissues, plants, yeast and bacteria. FOCUS™ Protease Arrest™ is compatible with 2D electrophoresis as it uses an alternative to EDTA as an inhibitor of metalloproteases. The absence of EDTA allows for optimal action of nucleases or removing nucleic acids from the samples. In addition, FOCUS™ ProteaseArrest™ uses PMSF as its primary serine protease inhibitor as opposed to the commonly used Pefabloc®. Pefabloc® has been reported to modify proteins at high concentrations and result in artifacts in subsequent 2D electrophoresis and mass spectrometry.

Sample Type & key features

Inhibition Specificity

1X Composition

Concentration & Available Sizes

For sensitive downstream proteomic applications (i.e. 2DGE and mass spec)

Reversible and irreversible inhibitors of serine, cysteine, calpain and metallo- proteases

340µM AEBSF

0.3µM Aprotinin

1.45µM Bestatin

3.4µM Calpain Inhibitor I

5.25µM Leupeptin

17µM Phosphoramidon

1mM PMSF

100X DMSO solution

1ml

Features

  •  2D electrophoresis compatible, broad spectrum protease inhibitor cocktail.
  •  100X concentrated, ready-to-use solution.
  •  High inhibition levels: 1X FOCUS™ ProteaseArrest™ inhibits >95% of protease activities (i.e. Mouse Pancreas Extract, 0.5mg/ml protein).
  • No proprietary ingredients, fully disclosed formulation of seven broad range protease inhibitors, including AEBSF, Aprotinin, Bestatin, Calpain Inhibitor I, Leupeptin, Phosphoramidon and PMSF

Applications

  •  Inhibition of protease activity in protein preparations.
  •  Suitable for 2D gel sample preparation for protection of protein samples.

ProteaseArrest Family

Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition. The ProteaseArrest™ family of inhibitor cocktails includes other species-specific inhibitor cocktails as well, all of which are enhanced with inhibitors specific for that species.  In addition to Bacteria, species-specific cocktails are available for BacterialPlantYeast, Fungus and Mammalian samples. Our Recom ProteaseArrest™ is designed for recombinant protein purification, particularly 6X His tagged proteins. These proteins are purified by IMAC (Immobilized Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA. Recom ProteaseArrest™ contains a specific bacterial metalloproteases that does not interfere with 6X His protein purification. The TCM ProteaseARREST™ protease inhibitor cocktail is a broad range, 200X concentrated, ready-to-use protease inhibitor cocktail sterile filtered for tissue culture media. TCM ProteaseArrest™ inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidases trypsin-like and acid proteases. Designed for the protection of proteins secreted in to the culture media.

Protocol
786-108F
Material Safety Data Sheet
786-108F
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
ProteaseArrestâ„¢ Protease Inhibitor Cocktail Offers Over 90% Inhibition of Proteases Improved protease inhibition compared to tablet cocktails  
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
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