FOCUS™ SubCell Kit (16 Citations)

Catalog
Description
Size
Price(USD)
Qty
Catalog
786-260
786-260
Description
FOCUS™ SubCell
FOCUS™ SubCell
Size
50 Preps
50 Preps
$458.00
$458.00
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FOCUS™ SubCell is designed for the total subcellular fractionation of cells and mammalian tissue into enriched fractions of nuclear, mitochondrial, cytosolic, and membrane proteins. The resulting mitochondrial fraction can be subsequently separated into heavy and light fractions by gradient centrifugation and the resulting mitochondria are >90% active and have intact inner and outer membranes. The kit includes reagents for optional steps that minimize contaminations of the nuclear fraction by cytoplasmic elements.
FOCUS™ SubCell is suitable for cultured animal cells and can be adapted for animal tissues. The kit is designed for 50 preps, where one prep is equivalent to 2x107 mammalian cells or 100mg mammalian tissue.
Features
- Adaptable for animal tissue
- Resulting fractions suitable for 2D electrophoresis and other applications
- Isolated mitochondria are ~90% active
Applications
-
Fractionation of cells and tissues into mitochondrial, nuclear, cytosolic and membrane proteins
-
Suitable for proteomic and cell signalling studies
Protocol | |
786-260 | ![]() |
Material Safety Data Sheet | |
786-260 |
Technical Literature | |
Enrichment of Nuclear, Mitochondrial, Cytosolic and Membrane Proteins with FOCUSâ„¢ SubCell | |
Mass Spectrometry Sample Prep Handbook | A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation. |
Plant Proteomics Handbook | |
Protein Electrophoresis Handbook | A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products. |
Sample Preparation Handbook | For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment |
- Liu, NK. et al (2022) Restoring mitochondrial homeostasis reduces cell death and promotes recovery after spinal cord injury. CELL DEATH DIS. https://doi.org/10.1038/s41419-022-05369-5
- Li, C. ET AL (2020) Sci Rep. doi.org/10.1038/s41598-020-63213-2
- Kosmider, B. et al (2019) Mitochondrial dysfunction in human primary alveolar type II cells in emphysema.doi.org/10.1016/j.ebiom.2019.07.063.
- Shiheido, Y. et al ( 2016) Porphyromonas gingivalis, a periodontal pathogen, enhances myocardial vulnerability, thereby promoting post-infarct cardiac rupture.J Mol Cell Cardiol. DOI: http://dx.doi.org/10.1016/j.yjmcc.2016.03.017
- Capel, F. et al (2015) J. Nutr. Biochem. doi:10.1016/j.jnutbio.2015.04.003
- Das, S. et al (2015) Sci Rep doi:10.1038/srep09759
- Hsu, E. et al (2015) Neoplasia. 17:497
- Dagda, R.K. et al (2014) J. Neurochem. 128:864
- Dodmane, P.R. et al (2014) Toxicol. Sci. 137:36
- Melendez, J. et al (2013) Gastroenterology. 145:808
- Stallings, N.R. et al (2013) PLOS. DOI: 10.1371/journal.pone.0071793
- Zhu, J.H. et al (2012) Cell Death Dis. 3:e312
- Bodas, M. et al (2011) J Immunol 186: 602
- Losel, R.M. et al (2010) PLOS. DOI: 10.1371/journal.pone.0009713
- Stallings, N.R. et al (2010) Neurobiol. Diss 40:404
- Titsworth, W. et al (2009) Glia. 57:1521