FTAC6

Fluorinated surfactants are unable to extract proteins from membrane, but are useful for membrane protein stabilization in subsequent purification steps as they do not strip natural lipids and other co-factors from the proteins. In addition, the bulky fluorinated tails can not penetrate into the interior and disrupt structure. Fluorinated surfactants often decrease non-specific aggregation and are thought to result in improved distribution on cryo-EM grids and better vitrification for cryo-EM data collection. They are also reported to increase crystallizability.