Fluoro™ Protease Assay

Fluoro™ Protease Assay

The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485nm and emission at 530nm to determine protease activity. The kit detects nanogram level of proteases present in the sample.

 

The kit is supplied with our chemically stabilized MSG-Trypsin™ for use as a general protease control; however, other specific protease standard controls can be used. MSG-Trypsin™ is an ultra-pure trypsin from porcine pancreas, modified by methylation followed by TPCK treatment and is extremely resistant to autolysis. The kit components are sufficient for 1,000 assays in a microtiter plate format.

 


Applications

  • Quantitative fluorescence protease assay

 

 

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Protocol
786-320
Material Safety Data Sheet
786-320
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Technical Literature
Bioassays Handbook
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
Catalog Description Size Price(USD) Qty
786-320
Fluoro™ Protease Assay 1000 Assays
$222.00