G-Trap™ rProtein A FF

G-Trap™ rProtein A FF are prepacked ready to use columns for purification of IgG antibodies.

The column is packed with fast flow resin, which is covalently coupled to recombinant Protein A. Recombinant Protein A is coupled covalently by proprietary coupling method to highly crosslinked 6% agarose. 

The recombinant Protein A, produced in E. coli, has been genetically engineered to enhance its binding capacity to the antibodies

G-Trap™ Protein G, 1 ml and G-Trap™ Protein G, 5 ml are also available for affinity purification of Immunoglobulins G.

The key characteristics of the recombinant Protein A resin or matrix used in G-Trap™ rProtein A FF  and the column properties are listed below

G-Trap™ rProtein A resin

• Mean particle size: 90 µM
• Bead Structure: Highly cross-linked 6% agarose
• Ligand: Recombinant Staphylococcal Protein A from E.coli
• Binding capacity:>40 mg Human IgG/ ml resin
• Recommended working flow rate:1 ml/min and 5 ml/min for 1 ml and 5 ml column respectively.
• Chemical Stability:Stable in all commonly used buffers
• pH stability: Long term: pH 3-9, short term pH 2-9
• Storage: 2°C to 8°C in 20% ethanol

G-Trap™ rProtein A, 1 ml columns

• Column Volume: 1 ml
• Columns Dimension: 0.7 x 2.5 cm
• Column Harware Pressure Limit: 0.5 MPa
• Column Hardware: Polypropylene (Biocompatible)

G-Trap™ rProtein A, 5 ml columns

• Column Volume: 5 ml
• Columns Dimension: 1.6 x 2.5 cm
• Column Harware Pressure Limit: 0.5 MPa
• Column Hardware: Polypropylene (Biocompatible)

Application (s): 

• G-Trap™ rProtein A FF are prepacked ready to use columns used for affinity purification of IgG antibodies.